食品科学 ›› 2006, Vol. 27 ›› Issue (1): 68-70.

• 基础研究 • 上一篇    下一篇

菌核侧耳β-葡萄糖苷酶产酶条件研究

包水明,李荣同,余志坚,陈传红   

  1. 东华理工学院生物系
  • 出版日期:2006-01-15 发布日期:2011-09-05

Study on Conditions of Producing β-glucosidase from Pleurotus tuber regium (Fr.) Sing

BAO Shui-ming,LI Rong-tong,YU Zhi-jian,CHEN Chuan-hong   

  1. Department of Biology, East China Institute of Technology
  • Online:2006-01-15 Published:2011-09-05

摘要: 通过单因素和正交试验,对菌核侧耳产β-葡萄糖苷酶液体培养条件进行优化。结果表明:以黄豆浆为氮源,葡萄糖为碳源,初始pH为6.0产酶条件最优。培养温度为25℃、装液量50ml/250ml、摇瓶转速为150r/min培养72h后,酶活力达最高,为1.86U/g(湿菌丝体)。

关键词: 菌核侧耳, &beta, -葡萄糖苷酶, 液体培养

Abstract: The optimum liquid culture conditions producing β-glucosidase from Pleurotus tuber regium (Fr.) Sing were studied. The optimum conditions for fermentation were: the soybean milk as the nitrogen source, the glucose as the carbon source, the initial optimum pH was 6.0. Under the optimal incubation condition: culture temperature was 25℃, volume 50ml/250ml, rotate speed 150r/min and culture time 72h, the highest β-glucosidase activity was obtained at wet weight of 1.86U/g (wet mycelium).

Key words: Pleurotus tuber regium (Fr.) Sing; liqiud culture; &beta, -glucosidase;