食品科学 ›› 2006, Vol. 27 ›› Issue (2): 108-111.

• 基础研究 • 上一篇    下一篇

桑叶中α—葡萄糖苷酶活性调节成分的研究

 马庆一, 时国庆, 陈春涛, 孙佳, 陈晓燕   

  1. 郑州轻工业学院食品与生物工程学院
  • 出版日期:2006-02-15 发布日期:2011-09-05

Studies on Inhibitor Effects and Activator of α-glucosidase in Mulberry Leaves

MA  Qing-Yi, SHI  Guo-Qing, CHEN  Chun-Tao, SUN  Jia, CHEN  Xiao-Yan   

  1. College of Food and Biological Engineering, Zhengzhou Institute of Light Industry
  • Online:2006-02-15 Published:2011-09-05

摘要: 从桑叶中提取、分离并部分纯化了多糖和黄酮。酶反应动力学研究结果表明,桑叶多糖是良好的α-葡萄糖苷酶竞争性抑制剂,其抑制率约比拜糖平片高8倍多,抑制率随浓度升高而增大,作用时间为5min时抑制效果最好。桑叶黄酮对α-葡萄糖苷酶有激活作用,是潜在的升糖功能因子。在桑叶黄酮加入乳化剂硬脂酸单甘酯,可改善黄酮在水中的分散度,解决了水溶性欠佳的黄酮酶活性调节剂的动力学测定问题。

关键词:  , &alpha, -葡萄糖苷酶抑制剂, 多糖, 黄酮, 糖尿病, 桑叶

Abstract: Polysaccharide and flavones were extracted, isolated, and partially purified from mulberry leaves. The results from studies by using enzymatic kinetics showed that, mulberry leave polysaccharide was an excellent competitive inhibitor of α- glucosidase for glucose releasing. Its inhibitory rate increased with increasing concentration. The optimum acting time for polysaccharide inhibition was 5min. Mulberry leave flavone was found as an activator of α-glucosidase, a potential functional factor for increasing blood sugar. Glycerol monostearicate, as an emulsifier, was introduced into the enzymatic reaction solvent system to enhance the homogeneity of flavones in water.

Key words:  , inhibitor of &alpha, -glucosidase; polysaccharide; flavones; diabetes; mulberry leaves;