食品科学 ›› 2007, Vol. 28 ›› Issue (1): 201-204.

• 生物工程 • 上一篇    下一篇

几种酶法从猪皮中提取胶原蛋白的对比研究

 王川, 李燕, 马志英, 蓝蔚青   

  1. 上海市食品研究所; 上海水产大学食品学院; 上海水产大学食品学院 上海200235; 上海200090; 上海200235;
  • 出版日期:2007-01-15 发布日期:2011-12-31

Comparison Study on Extraction of Collagen from Porcine Skin by Different Enzymes

 WANG  Chuan, LI  Yan, MA  Zhi-Ying, 蓝Wei-Qing   

  1. 1.Shanghai Food Research Institute, Shanghai 200235, China; 2.College of Food Science, Shanghai Fisheries University, Shanghai 200090, China
  • Online:2007-01-15 Published:2011-12-31

摘要: 研究了用胰酶、木瓜蛋白酶和胃蛋白酶从猪皮中提取胶原蛋白。研究发现,猪皮的蛋白质含量约为24.59%,羟脯氨酸含量为2.3%。猪皮经酶解处理后,即得到胶原蛋白粗提取液,粗提液再经过透析、浓缩,最后进行冷冻干燥,可得到纯度较高的胶原蛋白制品。研究结果显示:三种酶处理猪皮,其中以胰酶提取物得率最高,但结构破坏较严重,用胃蛋白酶提取的胶原蛋白制品的结构最完整,色泽最为洁白,但得率最低。

关键词: 胶原蛋白, 酶解, 羟脯氨酸, 提取

Abstract: This paper has studied the extraction of collagen from porcine skin by trypsin, papain and pepsin. There have been found to contain 24.59% protein, and 2.3% hydroxyproline, respectively of porcine skin. Collagen from porcine skin was extracted by three kinds of enzymes. After extraction it was purified further by dialyzing, condensing and lyophilizing. The results showed: Among the collagens extracted by three kinds of enzymes, the extraction rate of trypsin is the highest, but the structure of collagen is severely destroyed, while the structure of collagen by papain is the best, and also the whiteness was the highest among the collagens extracted, but the extraction rate is the lowest.

Key words: collagen, enzymatic hydrolysis, hydroxyproline, extraction