盐渍对双孢菇多糖的影响

食品科学 ›› 2007, Vol. 28 ›› Issue (10): 151-154.

盐渍对双孢菇多糖的影响

杨立红, 刘林德, 王晓洁, 屈慧鸽, 马秋慧, 蔡德华

鲁东大学生命科学学院；鲁东大学生命科学学院山东烟台264025；山东烟台264025；

出版日期:2007-10-15 发布日期:2011-11-22

Effects of Salting on Extraction of Agaricus bisporus Polysaccharides

YANG Li-Hong, LIU Lin-De, WANG Xiao-Jie, QU Hui-Ge, MA Qiu-Hui, CAI De-Hua

College of Biological Science and Technology, Ludong University, Yantai 264025, China

Online:2007-10-15 Published:2011-11-22

摘要/Abstract

摘要： 将盐渍和未盐渍的双孢菇分别经热水提取、乙醇沉淀得粗多糖,结果表明未盐渍双孢菇粗多糖的得率比盐渍双孢菇粗多糖高2倍。两份粗多糖经Sevag法除去蛋白质后上DEAE-纤维素柱层析,分别用蒸馏水和0.05mol/LNaCl溶液洗脱,得到多糖纯品组分Ab-I、Ab-I’和Ab-II、Ab-II’。SepharoseCL-4B测得盐渍与未盐渍双孢菇多糖的Ab-I和Ab-I’分子量均为7.79×104。红外光谱分析表明盐渍处理对双孢菇多糖结构未产生显著影响。多糖抗氧化能力测定的结果表明未盐渍双孢菇多糖的粗品和纯品的抗氧化能力均比盐渍双孢菇多糖的高。

关键词: 双孢菇, 盐渍蘑菇, 多糖, 分离鉴定, 抗氧化

Abstract: The salted and the not-salted Agaricus bisporus were exteacted by hot water and then deposited by ethanol. Determination results displayed that the extraction rate of crude polysaccharides of the not-salted Agaricus bisporus was 2 times higher than that of the salted. The DEAE cellulose column chromatography was used on polysaccharides purification after the protein was extracted with Seveg method. Pure polysaccharides namely Ab-I, Ab-I’ and Ab-II, Ab-II’ were isolated by distilled water and 0.05 mol/L solution of NaCl eluent respectively. The Sepharose CL-4B method showed that molecular weight of Ab- I and Ab-I’ were both 7.79 ×104. Infrared ray(IR) assays showed that the process of salting didn’t change the structure of Agaricus bisporus polysaccharides obviously. The determination results of anti-oxidant capacity of polysaccharides indicated that the anti- oxidant capacity of the crude and pure polysaccharides of not-salted Agaricus bisporus were both higher than that of the salted.

Key words: Agaricus bisporus, salted mushroom, polysaccharides, isolation and identification, anti-oxidation

杨立红, 刘林德, 王晓洁, 屈慧鸽, 马秋慧, 蔡德华. 盐渍对双孢菇多糖的影响[J]. 食品科学, 2007, 28(10): 151-154.

YANG Li-Hong, LIU Lin-De, WANG Xiao-Jie, QU Hui-Ge, MA Qiu-Hui, CAI De-Hua. Effects of Salting on Extraction of Agaricus bisporus Polysaccharides[J]. FOOD SCIENCE, 2007, 28(10): 151-154.

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