食品科学 ›› 2007, Vol. 28 ›› Issue (11): 386-390.

• 生物工程 • 上一篇    下一篇

分枝杆菌降解甾醇发酵生产雄烯二酮的两相系统培养基优化

 姜绍通, 胡锦艳, 杨英, 赵俊平, 崔福强   

  1. 合肥工业大学生物与食品工程学院; 合肥工业大学生物与食品工程学院 安徽合肥230009; 安徽合肥230009; 安徽合肥230009安徽建筑工业学院环境工程系; 安徽合肥230022;
  • 出版日期:2007-11-15 发布日期:2011-11-22

Medium Optimization of Androst-4-ene-3,17-dione Fermentation from Sterols by Mycobacterium sp. in Two-phase Systems

 JIANG  Shao-Tong, HU  Jin-Yan, YANG  Ying, ZHAO  Jun-Ping, CUI  Fu-Qiang   

  1. 1.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; 2.Department of Environment Engineering, Anhui Institute of Architecture, Hefei 230022, China
  • Online:2007-11-15 Published:2011-11-22

摘要: 对分枝杆菌Mycobacterium sp.BD696-6降解甾醇发酵生产雄烯二酮(AD)的两相系统培养基进行了优化。在甾醇添加量为0.6%时,培养基组成为有机相葵花油浓度20%、碳源糖蜜6%、氮源NH4NO30.3%、磷酸盐(NH4)2HPO40.08%条件下,AD得率最高,达到78.6%,比原培养基条件下得率提高了17.0%。

关键词: 雄烯二酮, 甾醇, 两相系统, 培养基优化

Abstract: The two-phase system medium for Mycobacterium sp.BD696-6 to produce Androst-4-ene-3,17-dione(AD)by degrading sterols was optimized. The obtained optimal medium ingredients were: sunflower oil 20%, molasses 6%, NH4NO3 0.3%, (NH4)2HPO4 0.08%. Under the optimal medium determined, the conversion rate reached to 78.6% when the concentration of substrate steroid was 0.6%, which is17.0% higher than the original medium.

Key words: Mycobacterium sp., sterols, two-phase system, medium optimization