食品科学 ›› 2007, Vol. 28 ›› Issue (2): 166-168.

• 生物工程 • 上一篇    下一篇

根癌农杆菌拮抗真菌的筛选、鉴定及发酵条件研究

 李祝, 宋宝安, 郁建平, 肖洋, 李颖, 梁宗琦   

  1. 贵州大学精细化工研究中心; 贵州大学生命科学学院; 贵州省产品质量检验检测院; 贵州大学生命科学学院 贵州贵阳550025; 贵州贵阳550025; 贵州贵阳550003;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Study on Screening, Identification and Fermentation Conditions of Antagonistic Fungi against Agrobacterium tumefacie

LI  Zhu, SONG  Bao-An, YU  Jian-Ping, XIAO  Yang, LI  Ying, LIANG  Zong-Qi   

  1. 1.Research and Development Center of Fine Chemicals, Guizhou University, Guiyang 550025, China; 2.College of Life Science, Guizhou University, Guiyang 550025, China; 3.Institute of Products Detection and Determination of Guizhou Province, Guiyang 550003, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 从多种植物根际土壤中分离到52株真菌菌株,通过琼脂柱法初筛及摇瓶复筛,筛选出1株对根癌农杆菌(Agrobacterium tumefaciens T-37)有明显抑制效果的菌株xj,经分类鉴定确定为黑曲霉(Aspergillus niger),并对之发酵条件进行了研究,结果表明:以2%果糖、3%牛肉膏为液体发酵培养基,10%装液量,起始pH6.0,25℃,140r/min,发酵5d,抑菌圈直径可达到35.28±0.32mm。

关键词: 根癌农杆菌, 黑曲霉, 筛选, 发酵

Abstract: 52 fungi strains were isolated from different rhizospheric soil samples, among which a strain xj to inhibit the growth of Agrobacterium tumefaciens T-37 was identified as Aspergilluse niger after being screened by agar column method and selected by liquid fermentation. The fermentation conditions for the Aspergilluse niger xj strain were optimized as follows: 2 % fructose, 3% beef extract as liquid ferment medium, 10% volume of substrate, initial pH6.0, 25℃,and 140r/min, for 5 days, while the inhibiting zones diameter was up to 35.28±0.32mm.

Key words: Agrobacterium tumefaciens, Aspergilluse niger, screen, fermentation