食品科学 ›› 2007, Vol. 28 ›› Issue (2): 229-231.

• 分析检测 • 上一篇    下一篇

乙酰胆碱酯酶生物传感器法测定蔬菜水果中有机磷农药残留

 魏福祥, 王振川, 王金梅   

  1. 河北科技大学环境科学与工程学院; 河北科技大学环境科学与工程学院 河北石家庄050018; 河北石家庄050018;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Determination of Organophosphorus Pesticide Residues by An Acetylcholinesterase Biosensor in Vegetables and Fruits

 WEI  Fu-Xiang, WANG  Zhen-Chuan, WANG  Jin-Mei   

  1. College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 研究一种快速简便测定蔬菜水果中有机磷农药残留的方法。利用乙酰胆碱酯酶生物传感器技术,以苹果、黄瓜为样品,采用标准加入法进行分析,测定蔬菜水果中有机磷农药残留。实验结果表明,该方法回收率在96.45%~100.12%,相对标准偏差为2.76%~3.09%,对马拉硫磷和甲基对硫磷的检出限分别为4.80×10-11、2.93×10-10mol/L。

关键词: 乙酰胆碱酯酶, 生物传感器, 标准加入法, 蔬菜水果, 有机磷农药残留

Abstract: A rapid testing method of organophosphorus pesticide residues in vegetables and fruits was studied in this paper. Using acetylcholinesterase biosensor technology, taking apples and cucumbers as examples, the organophosphorus pesticide residues were determined with standard addition method. The results showed that the rate of recovery is 96.45%~100.12%, and the relative standard deviation is 2.76%~3.09%. Detection limit of malation and methylparathion are 4.80×10-11, 2.93 ×10-10 mol/L respectively.

Key words: acetylcholinesterase, biosensor, standard addition method, vegetable and fruit, organophosphorus pesticide residues