食品科学 ›› 2007, Vol. 28 ›› Issue (2): 25-28.

• 基础研究 •    下一篇

电场对辣根过氧化物酶紫外-可见光谱及其活力指数的影响

 许强, 杨体强, 敖敦格日勒   

  1. 内蒙古大学理工学院; 内蒙古大学理工学院 内蒙古呼和浩特010021 内蒙古大学内蒙古自治区离子束生物工程重点实验室; 内蒙古呼和浩特010021; 内蒙古呼和浩特010021 内蒙古大学内蒙古自治区离子束生物工程重点实验室;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Effects of Electric Field Strengths on the Ultraviolet-Visible Spectra and Activity Index of Horseradish Peroxidase

 XU  Qiang, YANG  Ti-Qiang, AO  Dun-Ge-Ri-Le   

  1. 1.College of Science and Technology, Inner Mongolia University, Huhhot 010021, China;2.Key Laboratory of Ion Beam Bioengineering of Inner Mongolia Autonomous Region, Inner Mongolia University, Hohhot 010021, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 用不同电场强度处理辣根过氧化物酶,测试电场处理前后的紫外-可见光谱和酶的活力指数。结果表明:辣根过氧化物酶紫外-可见光谱发生了明显变化,说明电场可以改变酶的构象;一定电场强度可使酶活力指数增加,活力指数的增加可能是由酶构象的改变引起。研究结果对解释静电宏观生物效应具有重要意义。

关键词: 电场, 辣根过氧化物酶, 紫外-可见光谱, 构象, 活力指数

Abstract: After the horseradish peroxidase (HRP) was treated with different strengths of electric field, the HRP and activity index were assayed by ultraviolet-visible spectra. The results have shown that the HRP ultraviolet-visible spectra have been obviously changed accordingly. Experimental results also indicated that the conformation of HRP can be changed by electric field strengths and the activity index of HRP increase with certain strength of electric field. The increase of activity index might possibly be caused by the conformation changes of HRP. The results of this might have important meaning to further explore the macroscopical electrostatic biological effects.

Key words: electric field, horseradish peroxidase, ultraviolet-visible spectrum, conformation, activity index