食品科学 ›› 2007, Vol. 28 ›› Issue (6): 153-155.

• 工艺技术 • 上一篇    下一篇

碱溶性银耳粗多糖的提取及其清除自由基作用的研究

 吴琼, 郑成, 宁正祥, 刘本国, 董华强   

  1. 华南理工大学轻工与食品学院; 广州大学化学与化工学院; 佛山科学技术学院食品系 广东广州510640; 广东广州510091; 广东广州510640; 广东佛山528231;
  • 出版日期:2007-06-15 发布日期:2011-12-31

Study on Extraction and Antioxidant Effects of Crude Alkai-soluble Tremella Polysaccharide

WU  Qiong, ZHENG  Cheng, NING  Zheng-Xiang, LIU  Ben-Guo, DONG  Hua-Qiang   

  1. 1.College of Light Industry and Food Technology, South China University of Technology, Guangzhou 510640, China; 2.School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510091, China; 3.Department of Food Science, Foshan University, Foshan 528231, China
  • Online:2007-06-15 Published:2011-12-31

摘要: 本实验研究了碱溶性银耳粗多糖(crude alkai-soluble tremella polysaccharide,CATP)的提取及其清除自由基的作用。利用响应面软件进行实验设计,确定最佳提取方案为:提取时间3.56h,NaOH浓度0.76mol/L,液固比81.89。同时考察了碱溶性银耳粗多糖和水溶性银耳粗多糖(tremella polysaccharide,TP)对DPPH(二苯代苦肼基自由基)、羟基自由基、超氧自由基的清除作用。结果表明,碱溶性银耳粗多糖具有良好的抗氧化能力,并且随着多糖浓度的增高抗氧化性增强。

关键词: 银耳, 碱溶性粗多糖, 提取, 自由基

Abstract: It’s mainly studied on extraction and antioxidant effects on the role of crude alkai-soluble tremella polysaccharide(CATP). The optimum extracting conditions of CATP were as follows: extraction time 3.56 h, NaOH concentration 0.76 mol/L, solvent/solid ratio 81.89. The antioxidant effects of CATP and TP(tremella polysaccharide) were studied by scavenging DPPH, hydroxyl and superoxide anion free radical. The results showed that CATP has better antioxidant capabilities than TP.

Key words: Tremella fuciformis Berk, CATP, extraction, free radical