食品污染物苯并[a]芘致细胞损伤作用的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (7): 490-492.

食品污染物苯并[a]芘致细胞损伤作用的研究

宫智勇, 王耀峰, 刘良忠

武汉工业学院食品科技学院；武汉工业学院食品科技学院湖北武汉430023；湖北武汉430023；

出版日期:2007-07-15 发布日期:2011-10-24

Study on Damage of Cells Caused by Food Pollutant Benzo[a] Pyrene

GONG Zhi-Yong, WANG Yao-Feng, LIU Liang-Zhong

School of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China

Online:2007-07-15 Published:2011-10-24

摘要/Abstract

摘要： 目的:对食品污染物苯并[a]芘所致细胞损伤作用进行研究。方法:用不同浓度的苯并[a]芘(1、5、10、50、100μmol/L)对肺腺癌细胞A549染毒24h,MTT法测定细胞存活率、单细胞琼脂糖凝胶电泳检测细胞DNA损伤,用溶剂二甲基亚砜处理细胞组作为未染毒细胞组。结果:苯并[a]芘染毒作用时,随染毒浓度的增加,A549细胞存活率呈下降趋势;A549细胞的DNA损伤程度也随染毒浓度增加而增加,与未染毒A549细胞相比,染毒A549细胞的Olive尾矩值均有明显增加(p<0.05)。结论:食品污染物苯并[a]芘致A549细胞明显的损伤作用。

关键词: 食品污染物, 苯并[a]芘, 细胞损伤

Abstract: Objective: To investigate whether food pollutant benzo(a)pyrene causes the damage of A549 cells. Methods: A549 cells were exposed to different concentrations of benzo(a)pyrene (1, 5, 10, 50, 100 μmol/L BaP) for 24h. We measured the ability of viable cells by MTT assay, and detected DNA damage with alkaline single-cell gel electrophoresis. Results: We found A549 cells, which exposed to 1, 5, 10, 50, 100μmol/L BaP, had a significant decreased growth rate compared with the group not exposed to BaP (p< 0.05). And we found the dose-dependent increases in DNA damage in A549 cells exposed to BaP. The OTM values in A549 cells exposed to 1, 5, 10, 50, 100 μmol/L BaP all increased significantly compared with the group not exposed to BaP, respectively (p< 0.05). Conclusion: It is suggested food pollutant benzo [a] pyrene could cause the damage of A549 cells.

Key words: food pollutant, benzo(a)pyrene, cell damage

宫智勇, 王耀峰, 刘良忠. 食品污染物苯并[a]芘致细胞损伤作用的研究[J]. 食品科学, 2007, 28(7): 490-492.

GONG Zhi-Yong, WANG Yao-Feng, LIU Liang-Zhong. Study on Damage of Cells Caused by Food Pollutant Benzo[a] Pyrene[J]. FOOD SCIENCE, 2007, 28(7): 490-492.

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