关键词: 玉米蛋白粉, 酶解, 超滤, 功能特性, 生理活性

Abstract: In this paper,the proteins of corn gluten meal(CGM)were hydrolyzed by proteinase Alcalase and Protamex in order.Under room temperature,the hydrolysates were treated by ultrafiltration membranes of 20 kDa,10 kDa and 6 kDa cut-offs,and four fractions of ≥20 kDa,20～10 kDa,6～10 kDa and ≤6 kDa were obtained:The effect of different ultrafiltration pressure on functional properties and anti-oxidative activity of different molecular weight hydrolysates were studied.The results indicated that the yields of volumes and the proteins,the foam properties,and anti-oxidative activity of the hydrolysates were effected obviously by the pressure,while the viscosity and the emulsibility were not effected obviously.Under the pressure of 0.08 MPa,the functional properties and anti-oxidative activity of all of four fractions were better.The volume yield of the fraction of molecular weight ≤6 kDa was the highest.The protein yields of molecular weight of 6～10 kDa and ≥20 kDa were about 18%.The values of viscosity of all fractions were between 1.2～1.9 mPa·s,and the values of foam ability were between 148% and 207%,but the values of foam stability were lower.The emulsibility of the fraction of ≥20 kDa was the highest.The emulsion stability did not change obviously.The anti-oxidative activity of the fraction of ≤6 kDa was the highest.

Key words: CGM, hydrolysis, ultrafiltration, functional properties, biological activities