食品科学 ›› 2008, Vol. 29 ›› Issue (11): 391-394.

• 生物工程 • 上一篇    下一篇

酶法制备小麦麸皮戊聚糖的研究

 陈凤莲   

  1. 哈尔滨商业大学;
  • 出版日期:2008-11-15 发布日期:2011-12-08

Enzymatic Production of Pentosan from Wheat Bran

 CHEN  Feng-Lian   

  1. Harbin University of Commerce,Harbin 150076,China
  • Online:2008-11-15 Published:2011-12-08

摘要: 采用α-淀粉酶和碱性蛋白酶分别降解小麦麸皮中的淀粉和蛋白质成为可溶性组分,通过水洗过滤分离将其除去,以制得小麦麸皮戊聚糖。在淀粉的分离提取中,通过实验分析得出最佳参数为:料液比1:12(g/ml)、加酶量0.08g、酶解时间25min、酶解温度95℃。小麦麸皮中淀粉的含量从18.6%降至0.5%以下。在蛋白质的分离提取中,通过实验分析得出最佳参数为:酶解时间4h、酶解温度40℃、固液比5%(g/ml)、加酶量1.0%。酶解后小麦麸皮中蛋白质残留量仅为0.62%。经过去淀粉和蛋白质的小麦麸皮中戊聚糖含量为34.51%。

关键词: 戊聚糖, 淀粉, 蛋白质

Abstract: Starch and protein in wheat bran were degraded into soluble contents by α-amylase and alkaline protease respectively,and then the soluble contents were removed by water-washing method to produce pentosan.The optimal parameters for hydrolyzing starch are:material to liquid ratio 1:12(g/ml),addition of enzyme 0.08 g,time 25 min,and temperature 95 ℃.The remained content of starch is reduced to below 0.5 % from original 18.6 %.The optimal parameters for hydrolyzing protein are:time 4 h,temperature 40 ℃,solid to liquid ratio 5%(g/ml),addition of enzyme 1.0%.The remained content of protein in destarched wheat bran is only 0.62%.The destarched and deproteined wheat bran contents 34.51% pentosan.

Key words: pentosans, starch, protein