关键词: 粉绿铁线莲, 总黄酮, 提取

Abstract: Flavonoid exits in Clemat glauca Willd. In this study the total flavonoids contents were assayed by spectropho- tometry method with rutin as standard. The optimum extraction technologies of the total flavonoids from different parts of clematis glauca Willd. were obtained by orthogonal test. The results were as follows respectively: The best extraction conditions for stem are concentration of ethano1 70%, temperature 60℃, extraction time 2.5 h and rate of solid to solution 1:60, and the absorbance is 0.303; The best extraction conditions for leaf are concentration of ethanol 60%, temperature 60 ℃, extraction time 3.5h and rate of solid to soltion 1:30, and the absorbance is 0.751; The best extraction conditions for flower are concentration of ethanol 60%, rate of solid to solution 1:30, extraction time 2.5 h and temperature 60℃, and the absorbance is 0.950; The hest extraction conditions for furit are temperature 80 ℃, concentration of ethanol 60 %, rate of solid to solution 1:5 and extraction time 3 h, and the absorbance is 0.390.

Key words: Clematis glauca Willd, total flavonoids, extraction