食品科学 ›› 2008, Vol. 29 ›› Issue (9): 174-177.

• 工艺技术 • 上一篇    下一篇

复合酶法提取酸枣仁黄酮研究

 张明春, 解军波, 李婷, 陈衍芬, 刁瑞丽, 马古纯, 马占新, 瞿敬波   

  1. 天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室
  • 出版日期:2008-09-15 发布日期:2011-12-08

Extraction of Flavonoids from Seed of Wild Jujube by Complex Enzyme Method

 ZHANG  Ming-Chun, JIE  Jun-Bo, LI  Ting, CHEN  Yan-Fen, DIAO  Rui-Li, MA  Gu-Chun, MA  Zhan-Xin, QU  Jing-Bo   

  1. School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin Key Laboratory of Food Biotechnology,Tianjin 300134,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 以水提工艺为对照,研究了纤维素酶与果胶酶复合酶法提取酸枣仁黄酮新工艺。优化出最佳工艺条件:酶解温度为40℃,酶解液初始pH值为4.0,纤维素酶与果胶酶比为1:1,30U/g酸枣仁,酶解时间为5h,提取。复合酶工艺的酸枣仁黄酮提取率比传统水提工艺提高了54.67%。

关键词: 酸枣仁, 黄酮, 提取

Abstract: Objective: To increase extraction rate flavonoids from seed of wild jujube. Methods: The complex enzyme method and water extraction method of flavonoids were optimized respectively, and they were compares. Results: The optimal extraction conditions of flavonoids by complex enzyme method are hydrolysis temperture 40℃, pH of hydrolysis ratio of celluase to pectinase to pectinase 1:1, enzyme amount 30 U/g seed of wild jujube and hydrolysis time 5 h. Conclusion: The extraction rate of flavonoids by complex enzyme technology is 54.67% higher than that by traditional water exaction.

Key words: seed of wild jujube, flavonoids, extraction