食品科学 ›› 2008, Vol. 29 ›› Issue (9): 288-290.

• 工艺技术 • 上一篇    下一篇

豆粕中大豆异黄酮的超临界萃取工艺及性质研究

 潘利华, 罗建平   

  1. 合肥工业大学生物与食品工程学院;
  • 出版日期:2008-09-15 发布日期:2011-12-08

Study on Supercritical Fluid Extraction Technology and Characteristics of Isoflavones from Soy Bean Meal

 PAN  Li-Hua, LUO  Jian-Ping   

  1. Department of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 以脱脂豆粕为原料,通过正交试验,对超临界流体萃取法提取大豆异黄酮的工艺进行了优化研究,并测定了提取物的抗氧化性和抑菌性。实验结果表明,各因素对异黄酮提取率的影响程度由大到小依次为:萃取温度、萃取压力、夹带剂用量、萃取时间。抗氧化性研究表明,豆粕异黄酮浓度分别达1.0mg/ml和1.2mg/ml时,对·OH和O2·的清除率大于50%。抑菌性实验发现,豆粕异黄酮对金黄色葡萄球菌、枯草芽孢杆菌和地衣芽孢杆菌有较强的抑制作用。

关键词: 豆粕, 染料木素, 超临界流体萃取, 抗氧化, 抑菌

Abstract: The supercritical CO2 extraction technology of isoflavones from soy bean meal was potimized by orthogonal test,and the antioxidant and antimicrobial activeties of the isoflavones were determined. The experimental results showed that the order of different factors affecting extraction rate is extraction temperature>operation pressure>amount of modifier >extraction time. The scavenged rates of ·OH and O2· are both above 50% when the concentrations of the isoflavones are 1.0 mg/ml and 1.2 mg/ml, respectively. The products can inhibit the growth of Staphylococcus aureus, Bacillus subtilis and B.licheniformis.

Key words: soy bean meal, genistein, supercritical fluid extraction, antioxidant activity, antimicrobial activity