食品科学 ›› 2008, Vol. 29 ›› Issue (9): 423-426.

• 生物工程 • 上一篇    下一篇

有益元素Co和I对小麦胚芽愈伤组织VE积累的影响

 张恒, 李敏洁   

  1. 淮阴工学院生命科学与化学工程学院;
  • 出版日期:2008-09-15 发布日期:2011-12-08

Effects of Beneficial Elements Co and I on Accumulation of Vitamin E in Wheat Embryo Callus

 ZHANG  Heng, LI  Min-Jie   

  1. College of Life Science and Chemical Engineering,Huaiyin Institute of Technology,Huaian 223001,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 考查培养基中Co、I元素的含量及培养基初始pH对小麦胚芽愈伤组织VE积累的影响,建立VE积累和细胞生长的动力学模型。以次生代谢产物VE积累最多为目标,通过试验优化确定Co、I的含量和初始pH值的最佳组合。结果表明,当N6培养基中CoCl20.025mg/L、KI0.80mg/L、pH5.8时,有利于VE积累。VE积累的动力学模型的拟合度为98.11%,细胞增重的动力学模型拟合度为99.40%,非线性关系良好。有益元素Co和I对小麦胚芽愈伤组织VE积累有影响,I元素较Co元素的影响显著。

关键词: 小麦胚芽, VE积累, 有益元素, 非线性模型

Abstract: Besides contents of element Co and element I, the effect of initial pH in medium on accumulation of vitamin E (VE) for wheat embryo callus was studied. The mathematical models were established to describe growth kinetics and accumulation of vitamin E for wheat embryo callus cells. Based on the maximum accumulation of secondary metabolites vitamin E as an objective, the best combination of contents of Co and I with initial pH in medium was determined by orthogonal test. The results showed that the production of vitamin E in N6 medium is maxmium at the concentrations of CoCl2 0.025 mg/L and KI 0.80 mg/L as well as initial pH 5.8. The fitting degree of kinetic model of VE accumulation is 98.11%, and the fitting degree of kinetic model of cell growth is 99.40%, with good nonlinear relationship. The beneficial elements both Co and I affect accumulation of VE in medium for wheat germ callus, and the effect of the former is remarkabler than that of the later.

Key words: wheat embryo, accumulation of vitamin E, beneficial element, nonlinear models