食品科学 ›› 2008, Vol. 29 ›› Issue (9): 431-433.

• 生物工程 • 上一篇    下一篇

泡菜乳酸菌株的诱变选育

 刘月英, 赵士豪, 关中波   

  1. 河北经贸大学生物科学与工程学院; 河北省农林科学院粮油作物研究所;
  • 出版日期:2008-09-15 发布日期:2011-12-08

Mutation Screening of Lactobacillus for Production of Radish Pickle

 LIU  Yue-Ying, ZHAO  Shi-Hao, GUAN  Zhong-Bo   

  1. 1. College of Biological Science and Engineering,Hebei University of Economics and Business,Shijiazhuang 050061,China; 2. Cereal and Oil Crops Research Institute,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 首先采用紫外线和亚硝基胍对乳酸菌进行单因素诱变。确定紫外线照射剂量为150s,并得到一株产酸力为57.85°T的菌株,比原菌株产酸力提高了3.05%;NTG的诱变剂量是0.3mg/ml,处理时间为60min,得到产酸力为58.02°T的菌株,较出发菌株提高了6.52%。接着以紫外线和NTG对高产酸菌株进行了2轮复合诱变,最终得到产酸力达83.66°T的诱变株,较出发菌株产酸力提高了30.64%。

关键词: 乳酸菌, 诱变, 选育, 泡菜

Abstract: UV and NTG were used to mutate HET-4. Firstly, HET-4 was exposed in ultraviolet 150 s, obtaining a new strain. The acid-producing ability of the new strain is 57.85°T, higher 3.05 % than that of the original strain.When HET-S was disposed with 0.3 mg/ml NTG for 60 min, a new strain was obtained, and its acid-producing ability is 58.02°T, higher 6.52 % than before. The new strain was disposed with UV plus NTG for two times, obtaining a new strain HET-4-1, whose acid-producing ability is 83.66°T, higher 30.64 % than before.

Key words: Lactobacillus, mutation, screening, packle