食品科学 ›› 2010, Vol. 31 ›› Issue (7): 219-222.doi: 10.7506/spkx1002-6300-201007048

• 生物工程 • 上一篇    下一篇

从生抽酱醪中分离耐盐产酸酵母

杨秋明,郭彩华,蔡慧农   

  1. 集美大学生物工程学院
  • 收稿日期:2009-07-01 修回日期:2010-01-14 出版日期:2010-04-01 发布日期:2010-12-29
  • 通讯作者: 杨秋明 E-mail:yangqm@jmu.edu.cn
  • 基金资助:

    厦门市科技局资助项目(3502Z20071102)

Separation of Salt-tolerant and Acid-producing Yeasts from Soy Sauce Mash

YANG Qiu-ming,GUO Cai-hua,CAI Hui-nong   

  1. College of Bioengineering, Jimei University, Xiamen 361021, China
  • Received:2009-07-01 Revised:2010-01-14 Online:2010-04-01 Published:2010-12-29
  • Contact: YANG Qiu-ming E-mail:yangqm@jmu.edu.cn

摘要:

从发酵后期的生抽酱醪中分离得到一株产酸菌,根据溶钙圈、显微形态、菌落特征及生理与生化特征等确定其为可产酸酵母。在不同食盐质量浓度下测定该酵母的耗糖量以及生长量,可以发现该产酸酵母在22g/100mL 的食盐质量浓度条件下依然能够生长,并产生酸性物质。纸层析和气相色谱法表明该酵母的产酸类型为乳酸。随着食盐质量浓度的增大,发酵液的乳酸含量下降。当培养基中食盐质量浓度为16g/100mL 时,培养8d 后发酵液中乳酸含量为1.94%。

关键词: 耐盐产酸酵母, 乳酸, 溶钙圈, 酱醪

Abstract:

A strain with acid-producing ability identified based on calcium-dissolving ring, micro-morphology and colony characteristics, physiological and biochemical properties was isolated from late fermentation of soy sauce mash. Glucose consumption and growth of this strain were determined under various salt concentrations. This acid-producing yeast still grew well under the condition of 22 g/100 mL NaCl and produced acidic materials. Moreover, this strain was confirmed to be lactic acid type yeast by paper chromatography and gas chromatography. The increasing concentration of NaCl could result in the decrease of lactic acid content in fermentation broth. The lactic acid content was 1.94% (V/V) after 8 days of fermentation in the medium with 16 g/100 mL NaCl.

Key words: salt-tolerant and acid-producing yeast, lactic acid, calcium-dissolving ring, soy sauce mash

中图分类号: