食品科学 ›› 2010, Vol. 31 ›› Issue (9): 224-228.doi: 10.7506/spkx1002-6630-201009050

• 营养卫生 • 上一篇    下一篇

发酵乳杆菌SM-7的筛选及对小鼠降胆固醇作用

严玉婷1,潘道东1,2,*   

  1. 1.南京师范大学 国家乳品加工技术研发分中心 2.宁波大学生命科学与生物工程学院
  • 收稿日期:2009-09-07 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 潘道东 E-mail:daodongpan@163.com
  • 基金资助:

    国家“863”计划项目(2007AA10Z357);国家自然科学基金项目(30972130);
    国家农转基金项目(2009GB2C220412);江苏省科技支撑计划项目(BE2009366)

Screening and Hypocholesterolemic Effect of Lactobacillus fermentum

YAN Yu-ting1,PAN Dao-dong1,2,*   

  1. 1. Branch Center of National Dairy Products Processing Technology Development, Nanjing Normal University, Nanjing 210097,
    China;2. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
  • Received:2009-09-07 Online:2010-05-01 Published:2010-12-29
  • Contact: PAN Dao-dong E-mail:daodongpan@163.com

摘要:

筛选1 株具有高效降胆固醇活性的乳酸菌菌株并研究其降胆固醇的特性。采用分离自新疆酸马奶的10 株乳酸菌,通过益生活性的测定以及降胆固醇实验筛选得到1 株具有高效降胆固醇的益生菌株SM-7,经过鉴定为发酵乳杆菌。该菌株在液体培养基中的胆固醇脱除率为66.82%,用该菌液饲喂小鼠4 周,研究其对小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)以及体质量和肝、肾质量的影响。结果显示,实验组小鼠的血清TC、TG、LDL-C 含量均显著低于高脂模型组(P < 0.01),而HDL-C 含量没有显著差异(P > 0.05)。这表明发酵乳杆菌SM-7 对实验性高脂血症小鼠血清有显著的降胆固醇作用。

关键词: 发酵乳杆菌, 筛选, 降胆固醇, 作用机理

Abstract:

The strain SM-7 with hpocholesterolemic activity was screened from 10 lactic acid bacteria (LAB) strains isolated from koumiss in Xinjiang and its hpocholesterolemic effect in mice was investigated. The SM-7 was identified as Lactobacillus fermentum and could reduce 66.82% of cholesterol in the medium. The effect of strain SM-7 on serum TC, TG, HDL-C, LDLC, body weight, liver and kidney were investigated in ICR mice with hyperlipemia. Results showed serum TC, TG and LDL-C concentrations were exhibited a significant decrease (P < 0.01) while no obvious change was observed in serum HDL-C concentration (P > 0.05). These investigations suggested that Lactobacillus fermentum SM-7 strain had a significant effect on cholesterol-reducing function in mice with hyperlipemia.

Key words: Lactobacillus fermentum, screening, cholesterol-reducing, mechanism

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