真菌壳聚糖酶研究进展

摘要/Abstract

摘要： 壳聚糖酶是专一性降解壳聚糖的糖苷水解酶。在对比分析真菌壳聚糖酶和细菌壳聚糖酶的异同的基础上，总结近年来真菌壳聚糖酶在分类、酶学性质、基于分子生物学和生化工程的高产策略的研究进展。通过整理文献发现，来自真核微生物的壳聚糖酶降解产物为壳寡糖与氨基葡萄糖，具有分子质量大、活性较低等特点，针对酶活低这个迫切要解决的问题，讨论了提高产酶的潜在策略。

关键词: 真菌壳聚糖酶, 酶学性质, 高产策略

Abstract: Chitosanase is a glycoside hydrolase for specific degradation of chitosan. In this article, the recent research progress in the classification and characterization of chitosanases, and the current strategies for improving chitosanse activity are summarized. To the best of our knowledge and after an extensive literature review, we present that the degradation products by chitosanase from fungi are chitoligosaccharide and glucosamine with a high molecular weight and a low specific activity. Potential strategies for improving chitosanse activity are also discussed in this paper.

Key words: fungal chitosanase, enzymatic characteristics, high yield strategy

中图分类号:

Q819

龚香艺1,2，吴静1,2,*，邬敏辰2. 真菌壳聚糖酶研究进展[J]. 食品科学, 2012, 33(17): 308-311.

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