酪蛋白双酶水解物对直投式乳酸菌生长及发酵酸乳的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 258-263.

酪蛋白双酶水解物对直投式乳酸菌生长及发酵酸乳的影响

王佳佳，胡志和*

天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院

收稿日期:2012-04-25 修回日期:2012-10-23 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 胡志和 E-mail:huzhihe1008@yahoo.com.cn

Effect of Dual-Enzymatic Casein Hydrolysate on DVS Starter Growth and Yogurt Quality

Jia-Jia WANG,Zhi-He HU

Received:2012-04-25 Revised:2012-10-23 Online:2012-11-15 Published:2012-11-09
Contact: Zhi-He HU E-mail:huzhihe1008@yahoo.com.cn

摘要/Abstract

摘要： 以直投式乳酸菌为实验菌，研究富含ACE抑制活性肽的酪蛋白双酶水解物及其超滤物对乳酸菌的生长及发酵酸乳的影响。添加不同质量浓度的水解物和超滤物培养乳酸菌，检测对乳酸菌生长的影响，确定对乳酸菌生长无抑制作用的添加量，并将其应用于酸乳发酵。检测对酸乳的酸度、硬度、黏性、弹性等指标的的影响，结合感官评价，确定对酸乳的影响。结果表明：水解物的添加量为4g/100mL，分子质量在6000u以下的超滤物添加量为0.3g/100mL对乳酸菌的生长有促进作用，并且对发酵酸乳的感官质量无显著影响。水解物质量浓度≥5g/100mL、超滤物质量浓度≥0.35g/100mL对乳酸菌的生长起到抑制作用。

关键词: 酪蛋白, 双酶水解, 抑菌肽, 乳酸菌, 发酵乳

Abstract: An enzymatic casein hydrolysate rich in ACE inhibitory peptides was prepared through sequential hydrolysis with pepsin and trypsin and ultrafitrated to collect peptides less than 6000 u in molecular weight with ACE inhibitory activity. The effects of different concentrations of the casein hydrolysate and the peptides on the growth of DVS (Direct Vat Set) yogurt starter were explored. Their application in yoghurt was explored by investigating their effects on text properties such as acidity, hardness and viscosity and sensory quality. The casein hydrolysate when added at 4 g/100 mL and the peptides when added at 0.3 g/100 mL had a stimulating effect on the growth of lactic acid bacteria but did not have an obvious effect on the sensory quality of yoghurt. Inhibitory effects on the growth of lactic acid bacteria were observed when the hydrolysate was added at concentrations equaling or exceeding 5 g/100 mL or the peptides were added at concentrations equaling or exceeding 0.35 g/100 mL.

Key words: casein, dual-enzymatic hydrolysis, antibacterial peptide, lactic acid bacteria, yogurt

中图分类号:

TS252.4

王佳佳，胡志和* . 酪蛋白双酶水解物对直投式乳酸菌生长及发酵酸乳的影响[J]. 食品科学, 2012, 33(21): 258-263.

Jia-Jia WANG Zhi-He HU. Effect of Dual-Enzymatic Casein Hydrolysate on DVS Starter Growth and Yogurt Quality [J]. FOOD SCIENCE, 2012, 33(21): 258-263.

参考文献

[1] BRANTL V, TESCHEMACHER H, HENSCHEN A, et al. Novel opioid peptides derived from casein (beta -casomorphins). I. Isolation from bovine casein peptone[J]. Hoppe Seylers Z Physiol Chem, 1979, 360(9): 1211-1216.
[2] LOTTSPEICH F, HENSCHEN A, BRANTL V, et al. Novel opioid peptides derived from casein (beta-casomorphins). III. Synthetic peptides corresponding to components from bovine casein peptone. Hoppe Seylers Z Physiol Chem. 1980, 361(12): 1835–1839.
[3] MEISEL H, SCHLIMME E. Milk proteins: precursors of bioactive peptides[J]. Trends Food Science Technology. 1990, 1: 41-43.
[4] XU Ruojun. Bioactive peptides in milk and their biological and health implications[J]. Food Rev Int, 1998, 14(1): 1-9.
[5] 姜毓君, 李庆春, 闫宏博等. 乳蛋白生物活性肽的药理作用及应用前景[J]. 中国乳品工业, 2004, 32(5): 30-33.
[6] LAHOV E, REGELSON W. Antibacterial and immunostimulating casein-derived substances from milk: Casecidin, isracidin peptides[J]. Food And Chemical Toxicology, 1996, 34 (1): 131-145.
[7] LIEPKE C，ZUCHT H D, FORSSMANN W G, et al. Purification of novel peptide antibiotics from human milk[J]. Journal of Chromatography B, 2001, 752(2): 369-377.
[8] 胡志和, 区颜翠, 朱利民, 等. 酪蛋白水解物对瑞士乳杆菌生长的影响[J]. 食品科学, 2010, 31(23): 188-193.
[9] 夏庆, 薛正莲. 胰蛋白酶水解酪蛋白所得产物抗菌活性的研究[J]. 安徽工程科技学院学报, 2007, 22(1): 23-26.
[10] 王桂春, 吕兵. 分步酶解酪蛋白制备小分子ACE 抑制肽[J]. 食品科学, 2011, 32(21): 152-155.
[11] 洪伟, 薛正莲, 陈玲. 酪蛋白制备ACE抑制肽的酶解工艺优化[J]. 食品与发酵科技, 2010, 46(2): 37-40.
[12] 张艳, 胡志和, 闫星, 等. 胃蛋白酶水解酪蛋白制备ACE抑制肽的条件[J]. 食品科学, 2010, 31(14): 42-46.
[13] 申晓文, 牟光庆. 酪蛋白源ACE抑制肽的评价方法和研究进展[J]. 食品工业科技, 2010, 31(9): 375-381.
[14] CUSHMAN D W, CHEUNG H S. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung[J]. Biochemical Pharmacology, 1971, 20: 1637-1648.
[15] MIGUEL M., CONTETAS M M., RECIO I, et al. ACE-inhibitory and antihypertensive properties of a bovine casein hydrolysate[J]. Food Chemistry, 2009, 112(1): 211-214.
[16] 赵骏, 宫霞, 郭本恒. 乳酪蛋白源ACE抑制肽的分离纯化[J]. 中国乳品工业, 2006, 34(6): 8-11.
[17] 姜瞻梅, 田波, 霍贵成. 超滤法分离酪蛋白酶解物中的ACE抑制肽[J]. 食品与发酵工业, 2006, 32(10): 59-61.
[18] 廖敏. 低温长时间发酵酸奶加工关键技术与品质研究[D]. 雅安: 四川农业大学图书馆, 2005.
[19] 王微. 凝固型原味酸奶质地及微观结构的研究[D]. 哈尔滨: 东北农业大学图书馆, 2007.
[20] 谢继忠, 肖宏彬. 酸奶中乳酸菌数及酸度的检测与评价[J]. 中国乳品工业, 2002, 30(1): 23-25.
[21] RECIO, I. VISSER S. Identification of two distinct antibacterial domains with in the sequence of bovine aS2-casein. Biochimica et Biophysica Acta, 1999, 1428(2-3): 314-326.
[22] 李峦峰, 庞广昌, 阎亚丽. 酪蛋白来源新型抗菌肽中抗菌活性的初步研究[J]. 食品科学, 2003, 24(12): 34-36.
[23] 胡婷. 酪蛋白抗菌肽制备技术的研究[D]. 成都：西华大学, 2008.
[24] 侯恩娟. 酪蛋白抗菌肽的制备与研究[D]. 成都：西华大学, 2010.