L-精氨酸对肾脏组织形态和HSP70表达的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 271-274.

L-精氨酸对肾脏组织形态和HSP70表达的影响

张翠1,2，阮征2，印遇龙1,2，邓泽元2，吴信1,2,*

1.中国科学院亚热带农业生态研究所，中国科学院亚热带农业生态过程重点实验室 2.南昌大学生命科学与食品工程学院

收稿日期:2011-09-21 修回日期:2012-08-30 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 吴信 E-mail:w.xin@foxmail.com
基金资助:
国家自然科学基金;湖南省自然科学基金资助项目;食品科学与技术国家重点实验室开放基金资助

Effect of L-Arginine on Kidney Morphology and HSP70 Expression in Piglets

Xin WU

Received:2011-09-21 Revised:2012-08-30 Online:2012-11-15 Published:2012-11-09
Contact: Xin WU E-mail:w.xin@foxmail.com

摘要/Abstract

摘要： 目的：探讨L-精氨酸(L-Arg)对肾脏应激蛋白70(heat shock protein70，HSP70)表达量的影响，以及L-Arg对肾功能的保护作用机制。方法：以仔猪为模型，按照性别、体质量以及血缘一致原则随机分成2组：对照组、L-Arg组(基础日粮+0.6% L-Arg)。仔猪断奶后饲养7d，于第8天取血浆和肾脏组织，氨基酸分析仪测定血浆中的L-Arg、鸟氨酸(Orn)和瓜氨酸(Cit)。同时，荧光实时定量PCR测定肾脏中HSP70和诱导型一氧化氮合成酶(iNOS)mRNA表达水平，免疫印记(Western blotting)方法测定HSP70和iNOS的蛋白表达量，以及观察肾脏组织形态变化。结果：日粮中添加L-Arg可显著提高仔猪血浆中L-Arg和Orn的含量，同时减少肾小管中红细胞的数量和提高肾脏中iNOS和HSP70基因表达水平，以及HSP70的蛋白表达量。结论：在仔猪的日粮中添加L-Arg增加HSP70的蛋白和基因表达水平以及iNOS mRNA表达水平，维持肾脏蛋白构象的稳定，抑制应激引起的肾脏细胞凋亡途径的活化，减轻肾小管形态学损伤，避免断奶应激引起的肾脏损伤。

关键词: 精氨酸, 肾脏, 应激蛋白70, 仔猪

Abstract: Objective: To investigate the effect of L-arginine (L-Arg) on the expression of heat shock protein 70 mRNA and protein in kidney cells and its renal protective effect and mechanism. Methods: Twelve weaned piglets were randomly divided into two groups (6 piglets per group): one was control group and the other was L-Arg group, both of which were consistent with each other in terms of sex, body weight and consanguinity. A daily basal diet was fed to both groups and in addition, L-Arg was supplemented to the basal diet for the L-Arg group. After 7 d of feeding, all the piglets were killed, and plasma was collected for determination of the contents of L-Arg, ornithine (Orn) and citrulline (Cit) and kidney was also collected to determine the mRNA expression levels of HSP70 and inducible nitric oxide synthase (iNOS) by PCR and the protein expression levels of HSP70 and iNOS by Western blotting and to observe morphological changes. Results: L-Arg supplementation significantly increased plasma levels of L-Arg and Orn, mRNA expression levels of HSP70 and iNOS and HSP70 protein expression level and reduced the number of erythrocytes in kidney tubules. Conclusion: L-Arg supplementation to the diet of piglets can elevate HSP70 protein and mRNA expression and iNOS mRNA expression in the kidney and protect the kidney through maintaining the conformational stability of proteins, inhibiting the activation of apoptotic pathways of cells and alleviating kidney damage induced by weaning stress.

Key words: L-Arg, kidney, HSP70, piglet

中图分类号:

TS218
R151.3

张?翠1,2，阮?征2，印遇龙1,2，邓泽元2，吴?信1,2,*. L-精氨酸对肾脏组织形态和HSP70表达的影响[J]. 食品科学, 2012, 33(21): 271-274.

Xin WU. Effect of L-Arginine on Kidney Morphology and HSP70 Expression in Piglets[J]. FOOD SCIENCE, 2012, 33(21): 271-274.

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