食品科学 ›› 2012, Vol. 33 ›› Issue (22): 44-47.

• 工艺技术 • 上一篇    下一篇

响应面法优化菜籽饼粕多糖水酶法提取工艺

陈艳伟,王承明*   

  1. 华中农业大学食品科学技术学院,农业部食品安全评价重点开放实验室
  • 收稿日期:2011-08-22 修回日期:2012-10-08 出版日期:2012-11-25 发布日期:2012-11-20
  • 通讯作者: 王承明 E-mail:cmwang@mail.hzau.edu.cn
  • 基金资助:

    国家“十五”重大科技专项资助项目;湖北省重大科技攻关项目

Optimization of Aqueous Enzymatic Extraction of Polysaccharides from Rapeseed Meal by Response Surface Methodology

Yan-Wei CHEN,   

  • Received:2011-08-22 Revised:2012-10-08 Online:2012-11-25 Published:2012-11-20

摘要: 为优化菜籽饼粕多糖的水酶法提取工艺,在单因素试验的基础上,选择提取温度、提取时间、pH值以及加酶量为自变量,多糖得率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖得率的影响。利用SAS和响应面分析相结合的方法,模拟得到二次多项式回归方程的预测模型,并确定水酶法提取多糖最佳条件为加酶量256U/g、pH6.7、温度62℃、时间79min。在此条件下,多糖得率达到9.01%。

关键词: 响应面分析, 菜籽饼粕多糖, 提取, 优化

Abstract: The aqueous enzymatic extraction of polysaccharides from rapeseed meal was optimized by response surface methodology. One-factor-at-a-time method was employed to investigate the effects of five operating parameters on polysaccharide yield. The optimum ratio of water to material was 25:1 (mL/g). Further, a quadratic polynomial regression model describing polysaccharide yield as a function of enzyme dosage, pH, temperature and extraction time was developed using the SAS software based on a Box-Behnken experimental design. The results of response surface analysis showed that the optimal extraction conditions were extraction at 62 ℃ and pH 6.7 for 79 min with an enzyme dosage of 256 U/g, resulting in a polysaccharide yield as high as 9.01%.

Key words: response surface analysis, polysaccharides from rapeseed meal, extraction, optimization

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