芦笋下脚料乙醇提取物免疫功能及活性成分研究

食品科学 ›› 2013, Vol. 34 ›› Issue (1): 277-280.

芦笋下脚料乙醇提取物免疫功能及活性成分研究

田颖刚1，张盼1，黄宇玫2，王春艳1，朱胜1，乔娟娟1，谢明勇1,*

1.南昌大学食品科学与技术国家重点实验室 2.南昌大学科学技术学院

收稿日期:2011-11-09 修回日期:2012-12-19 出版日期:2013-01-15 发布日期:2013-01-07
通讯作者: 谢明勇 E-mail:xmync@sina.com
基金资助:
农业部公益性行业(农业)科研专项

Immunoregulatory Functions and Active Components of Ethanol Extract from Asparagus Scraps

Received:2011-11-09 Revised:2012-12-19 Online:2013-01-15 Published:2013-01-07

摘要/Abstract

摘要： 用75%乙醇为溶媒，超声提取芦笋下脚料，获得提取物，对提取物中总皂苷、总多糖、总游离氨基酸含量进行分析；用体外细胞培养法观察该提取物对正常及ConA诱导的小鼠脾淋巴细胞增殖的影响；以整体口服给药实验观察该提取物对正常小鼠免疫功能的影响，剂量分别为25、100、400mg/(kg·d)，连续给药7d。结果表明：该提取物总皂苷含量高达18.20%、总多糖及总游离氨基酸含量分别为14.86%和12.63%；体外培养对脾淋巴细胞增殖无影响，但对ConA诱导的脾淋巴细胞增殖呈低剂量促进高剂量抑制的作用；口服给药能增强小鼠碳廓清能力和ConA诱导的小鼠脾淋巴细胞转化增殖作用。芦笋下脚料乙醇提取物具有较明确的增强免疫功能的作用。

关键词: 芦笋提取物, 皂苷, 多糖, 游离氨基酸, 免疫功能

Abstract: In this study, 75% ethanol extract from asparagus scraps obtained by ultrasonic-assisted extraction was analyzed for the contents of total saponins, total polysaccharides and free amino acids. Meanwhile, its in vitro effect on the normal and Con A-induced proliferation of mouse spleen lymphocytes was studied, and the effect of oral administration at doses of 25, 100 mg/kg and 400 mg/kg on immune functions in normal mice was also observed. The ethanol extract contained 18.20% total saponins, 14.86% total polysaccharides and 12.63% free amino acids. It had no influence on normal spleen lymphocytes in vitro, whereas the ConA-induced proliferation of spleen lymphocytes could be promoted at 25 mg/kg but suppressed at 400 mg/kg by it. Oral administration of this extract enhanced carbon clearance capacity in mice and the ConA-induced proliferation of spleen lymphocytes. Therefore, this asparagus extract has remarkable immunoenhancing functions.

Key words: asparagus extract, saponin, polysaccharide, free amino acid, immune function

中图分类号:

TS201.4

田颖刚1，张?盼1，黄宇玫2，王春艳1，朱?胜1，乔娟娟1，谢明勇1,*. 芦笋下脚料乙醇提取物免疫功能及活性成分研究[J]. 食品科学, 2013, 34(1): 277-280.

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