食品科学

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利用伯克霍尔德氏菌脂肪酶富集裂壶藻油脂中的二十二碳六烯酸

兰 君1,2,宋晓金1,谭延振1,徐建春3,崔 球1,4,冯银刚1,*   

  1. 1.中国科学院青岛生物能源与过程研究所,青岛市单细胞油脂工程实验室,山东省能源生物遗传资源重点实验室,
    山东 青岛 266101;2.中国科学院大学生命科学学院,北京 100049;3.青岛琅琊台集团股份有限公司,
    山东 青岛 266400;4.中国科学院青岛生物能源与过程研究所,生物燃料重点实验室,山东 青岛 266101
  • 出版日期:2015-01-15 发布日期:2015-01-16

Concentration of Docosahexaenoic Acid in Aurantiochytrium sp. Oil by Burkholderia Lipase

LAN Jun1,2, SONG Xiaojin1, TAN Yanzhen1, XU Jianchun3, CUI Qiu1,4, FENG Yingang1,*   

  1. 1. Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of
    Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; 2. College of Life Sciences,
    University of Chinese Academy of Sciences, Beijing 100049, China; 3. Qingdao Langyatai Co. Ltd., Qingdao 266400, China;
    4. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology,
    Chinese Academy of Sciences, Qingdao 266101, China
  • Online:2015-01-15 Published:2015-01-16

摘要:

目的:研究伯克霍尔德氏菌胞外脂肪酶对裂壶藻油脂中二十二碳六烯酸(docosahexaenoic acid,DHA)的富集作用。方法:用三丁酸甘油酯平板筛选法,筛选产脂肪酶的细菌;以对硝基苯月桂酸酯为底物,对筛选得到的伯克霍尔德氏菌脂肪酶的酶学性质进行研究;用该脂肪酶水解裂壶藻油脂,通过气相色谱法测定酶解后水相和有机相的脂肪酸组成来研究其对裂壶藻油脂DHA的富集作用。结果:从广州的泥土样品中分离获得一株高产胞外脂肪酶的伯克霍尔德氏菌,在产酶培养基中30 ℃、200 r/min的条件下培养40 h后,脂肪酶酶活力达到最大值70 U/mL。脂肪酶粗酶液酶活性的最适温度为45 ℃,最适pH值为8.5。该菌的脂肪酶对裂壶藻油脂中的DHA有一定的富集作用,能够使油脂中DHA占总脂肪酸的质量分数从最初的30%升高到40%。结论:筛选得到一株能够产生选择性富集DHA的脂肪酶的细菌,拓宽了可用于多不饱和脂肪酸分离纯化的脂肪酶的范围。

关键词: 脂肪酶, 伯克霍尔德氏菌, 裂壶藻, 油脂, 二十二碳六烯酸富集

Abstract:

Purpose: To explore the effect Burkholderia lipase on enrichment of docosahexaenoic acid (DHA) in
Aurantiochytrium sp. oil. Methods: Tributyrin plate method was used to screen a lipase-producing bacterium. Using
4-nitrophenyl laurate as substrate, the enzymatic properties of the Burkholderia lipase were studied. The lipase was
used to hydrolyze Aurantiochytrium oil. To evaluate the enrichment efficiency of DHA with the lipase, the fatty acid
compositions in the aqueous and the organic phase after hydrolysis were detected by gas chromatography. Results: The
lipase-producing bacterium obtained from mud samples collected from Guangzhou city was identified as Burkholderia sp.
SD001. Its lipase activity reached the maximum value of 70 U/mL in the culture after fermentation at 30 ℃ for 40 h. The
lipase had an optimal pH of 8.5 and an optimal temperature of 45 ℃. When the lipase was used to hydrolyze the oil from
Aurantiochytrium sp., the percentage of DHA content in the oil was increased from 30% to 40%. Conclusions: A bacterium
producing a lipase capable of enriching DHA has been obtained. The lipase from this strain with expanded applicability is
useful for the enrichment of polyunsaturated fatty acid.

Key words: lipase, Burkholderia sp., Aurantiochytrium sp., oil, docosahexaenoic acid enrichment

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