食品科学 ›› 2018, Vol. 39 ›› Issue (13): 166-175.doi: 10.7506/spkx1002-6630-201813025

• 营养卫生 • 上一篇    下一篇

对虾原肌球蛋白不同致敏途径对BALB/c小鼠致敏性的影响

傅玲琳1,2,谢梦华1,王 翀1,王海燕2,王彦波1,2,*   

  1. 1.浙江工商大学食品与生物工程学院,浙江 杭州 310018;2.浙江食品质量安全工程研究院,浙江 杭州 310018
  • 出版日期:2018-07-15 发布日期:2018-07-09
  • 基金资助:
    国家自然科学基金面上项目(31571770)

Allergenicity of Shrimp Tropomyosin from Different Sensitization Approaches on BALB/c Mice

FU Linglin1,2, XIE Menghua1, WANG Chong1, WANG Haiyan2, WANG Yanbo1,2,*   

  1. 1. School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China; 2. Zhejiang Engineering Institute of Food Quality and Safety, Hangzhou 310018, China
  • Online:2018-07-15 Published:2018-07-09

摘要: 随着食物过敏现象的日益普遍,其已成为工业化国家一个严重的公众健康问题。食物蛋白致敏机理的相关 研究大多借助于两大类小鼠模型,即口服致敏模型和局部或皮肤致敏模型。然而,不同模型之间的差异以及如何选 择合适的模型进行研究却鲜有人关注。鉴于此,本研究旨在通过比较对虾原肌球蛋白口服灌胃和腹腔注射两种给药 途径对BALB/c小鼠致敏性的影响,寻求最佳给药方式,以便建立有效的动物模型,并在分子及免疫水平研究其致 敏机理,从而为食物过敏原动物模型的构建提供一定的理论依据,也为研究食物过敏原致敏机制以及预防治疗食物 过敏提供重要的模型依据。结果表明,腹腔注射小鼠血清中特异性免疫球蛋白(immunoglobulin,Ig)E、组胺以及 辅助性T细胞(helper T cells,Th)2型细胞因子含量均高于口服灌胃小鼠,其致敏性更好。此外,口服灌胃小鼠血 清特异性IgG2a、干扰素-γ、调节性T细胞水平增加,表明虽有黏膜佐剂作用,但口服给药仍可能使小鼠产生口服免 疫耐受,从而降低对虾原肌球蛋白对其的致敏性。本研究表明,腹腔注射原肌球蛋白比口服灌胃更易使小鼠致敏, 其Th1/Th2平衡被打破,Th2反应占据优势,更适合用于构建食物致敏动物模型。

关键词: 原肌球蛋白, 口服灌胃, 腹腔注射, 食物过敏, 小鼠模型

Abstract: Food allergy is an important public health issue in industrial countries due to the increasing prevalence and the potential life-threatening consequence. The understanding of food allergy mechanisms usually comes from experimental mouse models, which are broadly divided into two categories: oral sensitization model and topical or epicutaneous sensitization model. However, few studies have been focused on the difference between the two categories and the selection of the appropriate one. In this study, we aimed to develop a suitable route for tropomyosin administration by comparing the allergenicity of oral gavage and intraperitoneal injection in BALB/c mice in order to establish an effective animal model and to investigate the underlying mechanisms at the molecular and immunological levels, which will provide a theoretical basis for the establishment of animal model for food allergen research, the exploration of the mechanism of food allergen sensitization, and the prevention and treatment of food allergy. Results indicated that higher tropomyosin-specific immunoglobulin (Ig) E, histamine, and helper T cell (Th) type 2 cytokines were observed in intraperitoneally sensitized mice than those intragastrically sensitized, indicating that intraperitoneal injection was more sensitive in inducing systemic food allergy. Furthermore, higher levels of serum tropomyosin-specific IgG2a and interferon gamma, as well as regulatory T cell population were observed in intragastrically sensitized mice, suggesting that oral gavage may still develop oral tolerance to decrease the allergenicity of shrimp tropomyosin in BALB/c mice in spite of the presence of mucosal adjuvant. This experiment showed that intraperitoneal injection of tropomyosin to sensitized mice is easier than oral gavage, breaking the Th1/Th2 balance and making Th2 response dominant. Accordingly, intraperitoneal injection is more suitable to establish an animal model for food allergy research.

Key words: tropomyosin, oral gavage, intraperitoneal injection, food allergy, mouse model

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