基于超高效液相色谱-四极杆-飞行时间质谱法的兴安升麻甲醇提取物化学成分分析

doi:10.7506/spkx1002-6630-20180928-320

食品科学 ›› 2019, Vol. 40 ›› Issue (16): 169-176.doi: 10.7506/spkx1002-6630-20180928-320

基于超高效液相色谱-四极杆-飞行时间质谱法的兴安升麻甲醇提取物化学成分分析

吕弘，王晓明，刘小梅，张帆，王献瑞，郭亚卿，潘桂湘

1.天津中医药大学天津市现代中药重点实验室-省部共建国家重点实验室培育基地，天津 301617；2.天津中医药大学第二附属医院，天津 300250

出版日期:2019-08-25 发布日期:2019-08-26
基金资助:
国家自然科学基金面上项目（81173523）；国家自然科学基金青年科学基金项目（81303182）

Identification of Chemical Constituents of Methanol Extract of Cimicifuga dahurica by Ultra Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight-Tandem Mass Spectrometry

Lü Hong, WANG Xiaoming, LIU Xiaomei, ZHANG Fan, WANG Xianrui, GUO Yaqing, PAN Guixiang

1. Tianjin Municipal Key Laboratory of Modern Chinese Medicine-State Key Laboratory Breeding Base Co-founded by Province and Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; 2. Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China

Online:2019-08-25 Published:2019-08-26

摘要/Abstract

摘要： 目的：鉴定兴安升麻甲醇提取物中的化学成分。方法：采用超高效液相色谱仪分离兴安升麻甲醇提取物化学成分，四极杆-飞行时间质谱采集其一级和二级高分辨质谱数据，并用Masshunter软件对原始质谱数据进行分子特征提取，得到可能与兴安升麻相关的候选成分，根据化合物质谱裂解规律及部分标准品确证，鉴定化合物的结构。结果：共鉴定兴安升麻甲醇提取物中的38 个成分，包括苯丙素类、皂苷类，色原酮类、含氮化合物4 类成分。结论：超高效液相色谱-四极杆-飞行时间质谱可应用于升麻化学成分的鉴定。

关键词: 兴安升麻, 超高效液相色谱-四极杆-飞行时间质谱, 化学成分定性分析

Abstract: Objective: To identify the chemical components of the methanol extract from Cimicifuga dahurica. Methods: High-resolution mass spectrometric data of the extract were collected by ultra performance liquid chromatography coupled with quadrupole-time of flight-tandem mass spectrometry (UPLC-Q-TOF-MS). Through molecular feature extraction (MFE), Masshunter software was then used to process the original MS data. Several candidate compounds that may be derived from C. dahurica were obtained. Furthermore, the structures of these compounds were speculated according to their MS fragmentation patterns and confirmed by comparison with reference standards. Results: A total of 38 compounds were identified, including phenylpropanoids, saponins, chromones, and nitrogenous compounds. Conclusion: UPLC-Q-TOF-MS can be applied to identify the chemical components of C. dahurica.

Key words: Cimicifuga dahurica, ultra performance liquid chromatography coupled with quadrupole-time of flight-tandem mass spectrometry, qualitative analysis of chemical constituents

中图分类号:

吕弘，王晓明，刘小梅，张帆，王献瑞，郭亚卿，潘桂湘. 基于超高效液相色谱-四极杆-飞行时间质谱法的兴安升麻甲醇提取物化学成分分析[J]. 食品科学, 2019, 40(16): 169-176.

Lü Hong, WANG Xiaoming, LIU Xiaomei, ZHANG Fan, WANG Xianrui, GUO Yaqing, PAN Guixiang. Identification of Chemical Constituents of Methanol Extract of Cimicifuga dahurica by Ultra Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight-Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2019, 40(16): 169-176.

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基于超高效液相色谱-四极杆-飞行时间质谱法的兴安升麻甲醇提取物化学成分分析

Identification of Chemical Constituents of Methanol Extract of Cimicifuga dahurica by Ultra Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight-Tandem Mass Spectrometry

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