食品科学 ›› 2019, Vol. 40 ›› Issue (6): 128-135.doi: 10.7506/spkx1002-6630-20180117-232

• 生物工程 • 上一篇    下一篇

蜂房哈夫尼亚菌对粘质沙雷氏菌群体感应信号分子产生与生物被膜形成的调控

李婷婷1,高娜娜2,于海凤2,国竞文2,王当丰2,励建荣2,*,黄建联3   

  1. 1.大连民族大学生命科学学院,生物技术与资源利用教育部重点实验室,辽宁 大连 116600;2.渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁 锦州 121013;3.福建安井食品股份有限公司,福建 厦门 361022
  • 出版日期:2019-03-25 发布日期:2019-04-02
  • 基金资助:
    国家自然科学基金面上项目(31471639);“十三五”国家重点研发计划重点专项(2017YFD0400106)

Quorum Sensing Signal Molecule Production and Biofilm Formation in Serratia marcescens When Co-cultured with Hafnia alvei

LI Tingting1, GAO Nana2, YU Haifeng2, GUO Jingwen2, WANG Dangfeng2, LI Jianrong2,*, HUANG Jianlian3   

  1. 1. Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China; 2. Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, China; 3. Fujian Anjoy Food Share Co. Ltd., Xiamen 361022, China
  • Online:2019-03-25 Published:2019-04-02

摘要: 以腐败海鲈鱼中分离得到的2 株有群体感应现象的细菌为研究对象,经16S rRNA分子生物学鉴定其为蜂房哈夫尼亚菌(Hafnia alvei),命名为H. alvei-14;粘质沙雷氏菌(Serratia marcescens)产灵红素,命名为S. marcescens-01。研究不同培养条件下H. alvei-14与S. marcescens-01共培养后其信号分子N-酰基高丝氨酸内酯(N-acyl homoscrinc lactones,AHLs)分泌量和生物被膜产生量。结果表明:与H. alvei-14共培养能促进S. marcescens-01分泌AHLs和生物被膜的形成,这种影响可能与菌群间群体感应有关。环境条件的改变是影响细菌分泌AHLs和生物被膜形成的重要影响因子,较高盐浓度、pH值过低或过高均不利于微生物分泌AHLs和生物被膜形成,而限制性碳源及氮源等胁迫环境均可以刺激菌体群体感应系统,促进AHLs的释放和生物被膜的形成。

关键词: 蜂房哈夫尼亚菌, 粘质沙雷氏菌, 共培养, 群体感应

Abstract: The two bacterial strains with quorum sensing isolated from spoiled sea bass were identified based on 16S rRNA gene sequence analysis as Hafnia alvei-14 and Serratia marcescens-01 with the ability to produce prodigiosin, respectively. The production of the quorum-sensing signal molecules N-acyl homoserine lactones (AHLs) and biofilm formation in S. marcescens-01 were investigated when co-cultured with H. alvei-14 under different culture conditions. The results showed that co-culture with H. alvei could promote AHLs secretion and biofilm production in S. marcescens, which might be related to quorum sensing. Changes in environmental conditions were the important factors that could affect the secretion of AHLs and biofilm formation. Higher salt concentration and too low or too high pH values were unfavorable to microbial secretion of AHLs and biofilm formation, while restrictive carbon and nitrogen sources could stimulate the quorum sensing system and promote the release of AHLs and biofilm formation.

Key words: Hafnia alvei, Serratia marcescens, co-culture, quorum sensing

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