萝卜籽油中莱菔素的检测及加工工艺对莱菔素含量的影响

doi:10.7506/spkx1002-6630-20191015-142

食品科学 ›› 2020, Vol. 41 ›› Issue (20): 198-204.doi: 10.7506/spkx1002-6630-20191015-142

萝卜籽油中莱菔素的检测及加工工艺对莱菔素含量的影响

李楠楠，韩立娟，张维农，齐玉堂，贺军波，王青松，刘琛

（1.武汉轻工大学食品科学与工程学院，湖北武汉 430023；2.农产品加工与转化湖北省重点实验室，湖北武汉 430000）

出版日期:2020-10-25 发布日期:2020-10-23
基金资助:
农产品加工与转化湖北省重点实验室开放课题（2018HBSQGDKF04）；国家自然科学基金青年科学基金项目（31601505）；国家自然科学基金面上项目（31371783）

Detection of Sulforaphene Content in Radish Seed Oil and Influence of Processing Conditions on Sulforaphene Content

LI Nannan, HAN Lijuan, ZHANG Weinong, QI Yutang, HE Junbo, WANG Qingsong, LIU Chen

(1. School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2. Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan 430000, China)

Online:2020-10-25 Published:2020-10-23

摘要/Abstract

摘要： 通过对萝卜籽毛油样品前处理萃取方式的优化和加标回收实验，确定采用两步溶剂萃取方式检测萝卜籽油中莱菔素（sulforaphene，SFE）含量。考察萝卜籽预处理对SFE生成量的影响，以毛油中SFE含量为指标，分别用丙酮、二氯甲烷、乙醚、正己烷对其进行浸出法取油，并对比常规精炼与无水常温精炼工艺对油中SFE含量的影响。结果表明：用水和二氯甲烷进行两步萃取方式检测的萝卜籽毛油峰形好，响应值高，回收率和重复性较好。以SFE含量为指标，最佳的水分调节方式和预处理工艺是将萝卜籽进行粉碎后，直接喷洒调节水分质量分数到8%，25 ℃酶解10 min。最佳浸出溶剂为丙酮，浸出毛油中SFE含量高达832.36 mg/kg。采用常规精炼时，第1步水化脱胶后油中SFE就已完全损失。而采用无水常温精炼得到的萝卜籽油，SFE含量为760.35 mg/kg，保留率高达91.3%，同时，其基本指标均可达到国家三级食用油标准。本研究为萝卜籽油的加工和开发利用提供更多指导。

关键词: 萝卜籽油；莱菔素；加工工艺

Abstract: Through optimization of sample pretreatment conditions as well as spiked recovery experiments, a two-step extraction method was proposed to detect the sulforaphene (SFE) content in radish seed oil. The effect of radish seed pretreatment on SFE production was investigated. Acetone, dichloromethane, diethyl ether, and n-hexane were separately used to extract crude oil from pretreated radish seeds, in order to compare the effects of conventional refining and anhydrous room temperature refining on the retention rate of SFE in the oil. The results indicated that when detected using two-step extraction with water followed by dichloromethane radish seed oil showed good peak shape, high response value and good recovery and repeatability. The optimal water adjustment and pretreatment conditions that provided maximum SFE content were as follows: directly spraying crushed radish seeds with water to a moisture content of 8%, and then enzymatically hydrolyzing it at 25 ℃ for 10 min. Acetone was found to be the best extraction solvent, giving an SFE content of up to 832.36 mg/kg in the crude oil. SFE in the oil was completely lost after the first step of hydration degumming in the conventional refining process. However, the SFE content of the oil refined by anhydrous room temperature refining was 760.35 mg/kg, with a retention rate of SFE of up to 91.3%. Besides, the basic indexes of the refined oil could reach the national standard for the third grade of edible oil. This study provides useful guidance for the processing, development and utilization of radish seed oil.

Key words: radish seed oil; sulforaphene; processing steps

中图分类号:

TS224

李楠楠，韩立娟，张维农，齐玉堂，贺军波，王青松，刘琛. 萝卜籽油中莱菔素的检测及加工工艺对莱菔素含量的影响[J]. 食品科学, 2020, 41(20): 198-204.

LI Nannan, HAN Lijuan, ZHANG Weinong, QI Yutang, HE Junbo, WANG Qingsong, LIU Chen. Detection of Sulforaphene Content in Radish Seed Oil and Influence of Processing Conditions on Sulforaphene Content[J]. FOOD SCIENCE, 2020, 41(20): 198-204.

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萝卜籽油中莱菔素的检测及加工工艺对莱菔素含量的影响

Detection of Sulforaphene Content in Radish Seed Oil and Influence of Processing Conditions on Sulforaphene Content

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