食品科学 ›› 2020, Vol. 41 ›› Issue (4): 307-312.doi: 10.7506/spkx1002-6630-20181018-196

• 安全检测 • 上一篇    下一篇

谷物及其制品中脱氧雪腐镰刀菌烯醇及其衍生物的检测及污染规律分析

龚蕾,韩智,程慧,刘杰,彭青枝   

  1. (湖北省食品质量安全监督检验研究院,湖北省食品质量安全检测工程技术研究中心,湖北 武汉 430070)
  • 出版日期:2020-02-25 发布日期:2020-03-02
  • 基金资助:
    湖北省食品药品监督管理局科研项目(201601011;201802002)

Determination and Contamination Pattern of Deoxynivalenol and Derivatives in Cereals and Their Products

GONG Lei, HAN Zhi, CHENG Hui, LIU Jie, PENG Qingzhi   

  1. (Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Hubei Provincial Institute for Food Supervision and Test, Wuhan 430070, China)
  • Online:2020-02-25 Published:2020-03-02

摘要: 采用高效液相色谱-质谱联用技术建立测定小麦粉、挂面、玉米糁、大米等谷物及其制品中脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)、脱氧雪腐镰刀菌烯醇-3-葡萄糖苷(deoxynivalenol-3-glucoside,D3G)、3-乙酰基脱氧雪腐镰刀菌烯醇(3-acetyldeoxynivalenol,3ADON)及15-乙酰基脱氧雪腐镰刀菌烯醇(15-acetyldeoxynivalenol,15ADON)的检测方法,并对各种粮食中的污染情况进行调查。样品采用乙腈提取,正己烷除脂和固相萃取柱净化后,采用高效液相色谱-串联质谱检测,外标法定量。方法均经过优化和验证,满足谷物及其制品的检测要求。分析96 份谷物及其制品中4 种真菌毒素的污染规律,结果表明,15ADON无检出,只有1 个样品检出3ADON,DON和D3G均有不同程度的检出,DON检出率大于D3G,其中在挂面中的含量最高。D3G与DON的含量呈正相关,D3G/DON在30%左右,且不同谷物种类主要污染的毒素种类均以DON为主,其中挂面的污染最为严重。

关键词: 谷物及其制品, 高效液相色谱-串联质谱法, 脱氧雪腐镰刀菌烯醇, 衍生物, 污染规律

Abstract: High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been adopted to set up a method for detecting deoxynivalenol (DON), deoxynivalenol-3-glucoside (D3G), 3-acetyldeoxynivalenol (3ADON) and 15-acetyldeoxynivalenol (15ADON) in cereals and their products, such as wheat flour, noodles, rice and corn grit. Samples were extracted with acetonitrile and the extract was degreased with n-hexane and cleaned up on a solid-phase extraction (SPE) column prior to being detected by HPLC-MS/MS. The quantitation was performed using an external standard method. The method was optimized and validated to meet the requirements for the detection of grains and their products. Furthermore, we analyzed the pattern of contamination of the four mycotoxins in 96 samples of cereals and their products. The results showed that 15ADON was not detected in any sample and 3ADON was detected in only one sample. Both DON and D3G were found in many samples. The detection rate of DON was higher than that of D3G, and it was the highest in noodles. In addition, we found a positive correlation between the contents of D3G and DON. The D3G/DON ratio was generally around 30%. The major mycotoxin in all types of samples was DON, of which noodles were the most contaminated.

Key words: cereals and their products, high performance liquid chromatography-tandem mass spectrometry, deoxynivalenol, derivatives, contamination pattern

中图分类号: