食品科学 ›› 2022, Vol. 43 ›› Issue (3): 178-186.doi: 10.7506/spkx1002-6630-20201020-186

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水杨酸与硝普钠对采后芒果果实炭疽病抗性和苯丙烷代谢的协同诱导效应

何俊瑜,顾津羽,胡春梅,宋雅萍,田丹,肖桂云,任艳芳   

  1. (1.常州大学环境与安全工程学院,江苏 常州 213164;2.贵州大学农学院,贵州 贵阳 550025)
  • 出版日期:2022-02-15 发布日期:2022-03-08
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660477;31360413);常州大学引进人才项目(201709;201710)

Synergistic Effect of Combined Salicylic Acid and Nitric Oxide in Anthracnose Resistance and Phenylpropanoid Metabolism in Mango Fruit

HE Junyu, GU Jinyu, HU Chunmei, SONG Yaping, TIAN Dan, XIAO Guiyun, REN Yanfang   

  1. (1. School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China;2. College of Agriculture, Guizhou University, Guiyang 550025, China)
  • Online:2022-02-15 Published:2022-03-08

摘要: 为了明确水杨酸(salicylic acid,SA)和一氧化氮(nitric oxide,NO)在采后芒果炭疽病抗性诱导中的协同作用,以‘台农’芒果(Mangifera indica L.)为材料,分析了2 mmol/L SA和0.25 mmol/L NO供体硝普钠(sodium nitroprusside,SNP)单独和复合处理对果实接种炭疽病原菌(Colletotichum gloeosporioides)后发病率、病斑直径及抗病相关酶活力、抗病物质含量的影响。结果表明,SA和SNP单独或复合处理均明显降低了果实炭疽病斑的生长,降低了果实丙二醛的积累,减缓了炭疽病的发病进程,且以SA+SNP处理的效果更明显。SA、SNP和SA+SNP处理均明显提高了果实中抗病相关酶(苯丙氨酸转氨酶、肉桂醇脱氢酶、肉桂酸-4-羟化酶、对香豆酰-CoA连接酶、过氧化物酶、多酚氧化酶、几丁质酶、β-1,3-葡聚糖酶)活力,增加了果实体内木质素、总酚和类黄酮的积累,且以SA+SNP处理作用最为明显。结论:SA和SNP在诱导果实抗病反应中具有协同增效的作用,该研究为降低由炭疽病菌侵染引起的病害及采后芒果果实防腐保鲜提供了参考。

关键词: 芒果;抗病;水杨酸;一氧化氮;协同作用

Abstract: To clarify the synergistic effect between salicylic acid (SA) and nitric oxide (NO) in inducing resistance against anthracnose in postharvest mango fruit, the effects of single and combined treatment with 2 mmol/L SA and 0.25 mmol/L sodium nitroprusside (SNP, a NO donor) were investigated on the disease incidence, lesion diameter, and disease resistance-related enzyme activities as well as substance contents in ‘Tainong’ mango fruit (Mangifera indica L.) inoculated with Colletotichum. gloeosporioides. The results showed that SA, SNP and their combination significantly reduced the growth of C. gloeosporioides, decreased malondialdehyde (MDA) accumulation, and slowed down the progression of anthracnose. In addition, the activities of resistance-associated enzymes, such as phenylalanine ammonia lyase (PAL), cinnamyl alcohol dehydrogenase (CAD), cinnamate-4-hydroxylase (C4H), 4-coumarate coenzyme A ligase (4CL), polyphenol oxidase (PPO), peroxidase (POD), chitinase (CHI) and β-1,3-glucanase (GLU) were increased markedly, and the accumulation of lignin, total phenols and flavonoids was elevated. The combination of SA and SNP was more effective than either alone. The above results show that exogenous SA and SNP combination synergistically protects mango fruit from fungal infection and improves fruit resistance to postharvest diseases, which can provide useful information for reducing the disease caused by invasion of C. gloeosporioides and preserving postharvest mango fruit.

Key words: mango; disease resistance; salicylic acid; nitric oxide; synergistic effect

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