食品科学 ›› 2024, Vol. 45 ›› Issue (24): 250-258.doi: 10.7506/spkx1002-6630-20240510-074

• 安全检测 • 上一篇    下一篇

基于CeO2/CoOOH纳米酶的比色分析法快速检测水果中抗坏血酸

岳晓月,吴朝云,闫曙亮,符龙,赵电波   

  1. (1.郑州轻工业大学食品与生物工程学院,河南 郑州 450001;2.冷链食品加工与安全控制教育部重点实验室,河南 郑州 450001;3.河南省冷链食品质量安全控制重点实验室,河南 郑州 450001)
  • 出版日期:2024-12-25 发布日期:2024-12-06
  • 基金资助:
    河南省重点研发与推广专项(科技攻关)(232102111061;222102310355);河南省重大公益专项(201300110100)

Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits

YUE Xiaoyue, WU Chaoyun, YAN Shuliang, FU Long, ZHAO Dianbo   

  1. (1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China; 2. Key Laboratory of Cold Chain Food Processing and Safety Control, Ministry of Education, Zhengzhou 450001, China; 3. Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China)
  • Online:2024-12-25 Published:2024-12-06

摘要: 本研究采用液相沉淀法合成了一种二氧化铈(CeO2)修饰羟基氧化钴(CoOOH)的纳米材料CeO2/CoOOH。CeO2/CoOOH具有良好的类氧化酶活性,可以直接氧化3,3’,5,5’-四甲基联苯胺(3,3’,5,5’-tetramethylbenzidine,TMB)为蓝色的氧化态-TMB(ox-TMB),而抗坏血酸(ascorbic acid,AA)的强还原性可以将ox-TMB还原为TMB,导致ox-TMB在652 nm波长处的吸光度降低,同时蓝色褪去。在此基础上开发了一种新的检测AA的比色传感器。结果表明:该传感器显示出对AA的高灵敏度响应性,检测限为2.73 μg/mL,检测范围为5~100 μg/mL,可成功应用于橙子和苹果样品中AA的快速检测。

关键词: 快速检测;比色法;抗坏血酸;纳米酶

Abstract: In this study, cerium dioxide-modified cobalt oxyhydroxide (CeO2/CoOOH) nanomaterial was synthesized by a liquid-phase precipitation method. CeO2/CoOOH showed good oxidase-like activity toward 3,3’,5,5’-tetramethylbenzidine (TMB), and could directly oxidize TMB to ox-TMB, which presented a clear blue color. Reversely, ascorbic acid (AA) could reduce ox-TMB to TMB, leading to a decrease in the absorbance of ox-TMB at 652 nm and a fading of the blue color. Based on this phenomenon, a new colorimetric sensor for AA detection was developed. The sensor exhibited a highly sensitive response to AA with a limit of detection (LOD) of 2.73 μg/mL and a wide detection range of 5-100 μg/mL and could be successfully applied for rapid detection of AA in oranges and apples.

Key words: rapid detection; colorimetric method; ascorbic acid; nanozyme

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