利用电化学阻抗谱快速评估冰藏早期大黄鱼肌肉细胞状态与品质劣变

doi:10.7506/spkx1002-6630-20251226-223

食品科学 ›› 2026, Vol. 47 ›› Issue (12): 329-336.doi: 10.7506/spkx1002-6630-20251226-223

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利用电化学阻抗谱快速评估冰藏早期大黄鱼肌肉细胞状态与品质劣变

祁亚，杨敏，梁艳，高元沛，赵永强，金仁耀，周勇，张小军，焦龙，张微，邓尚贵，袁鹏翔

（1.浙江海洋大学食品与药学学院，浙江舟山 316000；2.中国水产科学研究院南海水产研究所，农业农村部水产品加工重点实验室，国家水产品加工技术研发中心，广东广州 510300；3.三亚热带水产研究所，海南省深远海渔业资源高效利用与加工重点实验室，海南三亚 572018；4.浙江工商大学海洋食品研究院，浙江杭州 310012；5.舟山市食品药品检验检测研究院，浙江舟山 316012；6.浙江省海洋水产研究所，浙江舟山 316021）

发布日期:2026-07-08
基金资助:
国家自然科学基金青年科学基金项目（32302203）；浙江省“尖兵”“领雁”研发攻关计划项目（JB2026006）；舟山市关键和共性技术攻关项目（2025C03007）

Rapid Evaluation of Cellular Status and Quality Deterioration in Larimichthys crocea Muscle during Early Ice Storage Using Electrochemical Impedance Spectroscopy

QI Ya, YANG Min, LIANG Yan, GAO Yuanpei, ZHAO Yongqiang, JIN Renyao, ZHOU Yong,ZHANG Xiaojun, JIAO Long, ZHANG Wei, DENG Shanggui, YUAN Pengxiang

(1. College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China; 2. National R&D Center for Aquatic Product Processing, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 3. Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572018, China; 4. Institute of Sea Food, Zhejiang Gongshang University, Hangzhou 310012, China; 5. Zhoushan Institute for Food and Drug Inspection and Testing, Zhoushan 316012, China; 6. Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, China)

Published:2026-07-08

摘要/Abstract

摘要： 为实现冰藏早期大黄鱼肌肉细胞状态与品质的精准评估，本研究采用电化学阻抗谱（electrochemical impedance spectroscopy，EIS）技术，通过等效电路模型拟合提取细胞膜电容（capacitance of cell membrane，Cm）、细胞外液电阻（extracellular resistance，Re）和细胞内液电阻（intracellular resistance，Ri）等特征参数，并分析其与鱼类关键生化指标（三磷酸腺苷（adenosine triphosphate，ATP）含量、乳酸脱氢酶活性、pH值）及常规品质指标（水分活度、持水性、肌肉收缩率、质构特性）的相关性。结果表明，冰藏前期（0～4 h）细胞ATP快速消耗引发代谢紊乱，导致细胞肿胀和强烈收缩，Cm、Re和Ri表现为同步上升，同时持水性急剧下降、硬度增加；冰藏后期（4～24 h）随着ATP耗竭和细胞膜完整性破坏，肌肉细胞Cm和Re缓慢下降、Ri持续升高，同时持水性稳定在较低水平，出现肌肉软化等不可逆品质劣变。综上，EIS特征参数能够灵敏捕捉基于常规生化与物理指标所反映的品质变化关键节点，可为鱼体品质的无损实时监测提供基于细胞电生理状态的理论依据。

关键词: 大黄鱼；冰藏早期；电化学阻抗谱；肌肉细胞状态；品质

Abstract: To achieve precise evaluation of cellular status and quality in Larimichthys crocea muscle during early ice storage, this study employed electrochemical impedance spectroscopy (EIS). The characteristic parameters including capacitance of cell membrane (Cm), extracellular resistance (Re) and intracellular resistance (Ri) were extracted by equivalent circuit modeling. Their correlations with key biochemical indicators (adenosine triphosphate (ATP) content, lactate dehydrogenase activity, and pH) and routine quality indicators (water activity, water holding capacity (WHC), muscle contraction rate, and texture) were analyzed. Results indicated that during the early stage of ice storage (0–4 h), rapid ATP consumption in cells induced metabolic disorders, causing cellular swelling and intense contraction. This was manifested as simultaneous increases in Cm, Re, and Ri, accompanied by a sharp decline in water holding capacity and increased hardness. In the later stage (4–24 h), with the depletion of ATP and the disruption of cell membrane integrity, Cm and Re decreased while the Ri continued to rise; WHC stabilized at low levels, accompanied by irreversible quality deterioration such as muscle softening. In summary, EIS characteristic parameters can sensitively capture critical junctures of quality changes reflected by routine biochemical and physical indicators, providing a theoretical foundation for non-destructive and real-time monitoring of fish quality based on cellular electrophysiological status.

Key words: Larimichthys crocea; early ice storage; electrochemical impedance spectroscopy; muscle cellular state; quality

中图分类号:

TS254.7

祁亚，杨敏，梁艳，高元沛，赵永强，金仁耀，周勇，张小军，焦龙，张微，邓尚贵，袁鹏翔. 利用电化学阻抗谱快速评估冰藏早期大黄鱼肌肉细胞状态与品质劣变[J]. 食品科学, 2026, 47(12): 329-336 https://doi.org/10.7506/spkx1002-6630-20251226-223

QI Ya, YANG Min, LIANG Yan, GAO Yuanpei, ZHAO Yongqiang, JIN Renyao, ZHOU Yong,ZHANG Xiaojun, JIAO Long, ZHANG Wei, DENG Shanggui, YUAN Pengxiang. Rapid Evaluation of Cellular Status and Quality Deterioration in Larimichthys crocea Muscle during Early Ice Storage Using Electrochemical Impedance Spectroscopy[J]. FOOD SCIENCE, 2026, 47(12): 329-336 https://doi.org/10.7506/spkx1002-6630-20251226-223

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利用电化学阻抗谱快速评估冰藏早期大黄鱼肌肉细胞状态与品质劣变

Rapid Evaluation of Cellular Status and Quality Deterioration in Larimichthys crocea Muscle during Early Ice Storage Using Electrochemical Impedance Spectroscopy

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