L-茶氨酸缓解短期高盐饮食暴露所致C57BL/6J小鼠肾脏纤维化

doi:10.7506/spkx1002-6630-20260511-060

食品科学 ›› 2026, Vol. 47 ›› Issue (19): 1-11.doi: 10.7506/spkx1002-6630-20260511-060

• 特色药食同源资源与人类健康专栏 •

L-茶氨酸缓解短期高盐饮食暴露所致C57BL/6J小鼠肾脏纤维化

唐芳，唐心月，周林灵，梁婷婷，陈颖，王永怡，宋家乐

（1.桂林医科大学公共卫生学院，广西桂林 541199；2.梧州医学高等专科学校，广西梧州 543199；3.广西环境暴露组学与全生命周期健康重点实验室，广西桂林 541199；4.桂林医科大学检验与生物技术学院，广西桂林 541004；5.广西高校医药生物技术与转化医学重点实验室，广西桂林 541199）

发布日期:2026-10-22
基金资助:
中央引导地方科技发展基金专项（桂科ZY22096025）；广西壮族自治区大学创新创业训练计划项目（202210601038）；广西壮族自治区八桂青年拔尖人才培养项目（GCHH〔2024〕55）

L-Theanine Alleviates Renal Fibrosis Induced by Short-Term High-Salt Diet Exposure in C57BL/6J Mice

TANG Fang, TANG Xinyue, ZHOU Linling, LIANG Tingting, CHEN Ying, WANG Yongyi, SONG Jiale

(1. School of Public Health, Guilin Medical University, Guilin 541199, China; 2. Wuzhou Medical College, Wuzhou 543199, China; 3. Guangxi Key Laboratory of Environmental Exposomics and Whole Life Cycle Health, Guilin 541199, China; 4. School of Laboratory Medicine and Biotechnology, Guilin Medical University, Guilin 541004, China; 5. Guangxi University Key Laboratory of Medical Biotechnology and Translational Medicine, Guilin 541199, China)

Published:2026-10-22

摘要/Abstract

摘要： 目的：探讨L-茶氨酸（L-theanine，LT）对2 周高盐饮食（8% NaCl组）暴露下小鼠肾脏纤维化的保护作用。方法：将28 只4 周龄SPF级C57BL/6J雄性小鼠随机分为对照组和实验组。定时记录小鼠体质量、摄食量、饮水量、血压和心率变化。测定血清肌酐和白细胞介素-1β（interleukin-1β，IL-1β）、IL-6和肿瘤坏死因子-α水平。采用苏木精-伊红染色和过碘酸-雪夫染色观察肾脏组织学变化，采用Masson和天狼星红染色评估肾脏纤维化程度；检测肾脏组织中Kim1、Vim、Acta2、Smad3及Cdh1的mRNA表达水平，以及E-cadherin、Kim-1、CTGF的蛋白表达水平。结果：LT干预能够显著降低8% NaCl组小鼠血清IL-1β和IL-6水平（P＜0.05）。Masson和天狼星红染色显示，LT干预组小鼠的肾小球及肾间质纤维化程度均显著减轻（P＜0.05）。LT干预可显著上调8% NaCl所致肾脏组织中E-cadherin的mRNA和蛋白表达水平的下降（P＜0.05），并显著抑制Vim、Acta2、Smad3等mRNA表达（P＜0.05）。与8% NaCl组相比，LT处理还可上调E-cadherin蛋白表达水平，同时下调α-SMA的蛋白表达水平（P＜0.05），减少肾间质纤维组织堆积，显著改善肾脏组织纤维化。结论：茶氨酸干预可减轻高盐饮食诱导的小鼠肾脏炎症反应，延缓肾脏纤维化病变，其保护机制可能与调控E-cadherin、Kim-1、Vimentin及α-SMA的表达有关。

关键词: 短期高盐；肾脏损伤；L-茶氨酸；肾脏纤维化

Abstract: Objective: To investigate the protective effect of L-theanine (LT) against renal fibrosis in mice exposed to a high-salt diet (HSD, 8% NaCl) for 2 weeks. Methods: A total of 28 four-week-old SPF-grade C57BL/6J male mice were randomly divided into four groups: control, HSD, HSD + low-dose L-theanine (LLT, 10 mg/kg), and HSD + high-dose L-theanine (HLT, 50 mg/kg). Body mass, food intake, water intake, blood pressure, and heart rate were recorded periodically. Serum creatinine (SCr) and inflammatory cytokine levels, including interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), were determined. Hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining were used for renal histopathological evaluation, and Masson’s trichrome and Sirius red staining were used for the assessment of renal fibrosis. The mRNA expression of the genes encoding kidney injury molecule-1 (Kim1), vimentin (Vim), α-smooth muscle actin (Acta2), Smad family member 3 (Smad3), and E-cadherin (cdh1), as well as the protein expression of E-cadherin, Kim-1, and connective tissue growth factor (CTGF), was analyzed. Results: LT intervention reduced serum IL-1β and IL-6 levels in the HSD group (P < 0.05). Masson’s trichrome and Sirius red staining showed that glomerular and interstitial fibrosis were significantly reduced in the LT intervention groups (P < 0.05). LT intervention significantly upregulated the mRNA and protein expression of E-cadherin in kidney tissues, which were decreased by HSD (P < 0.05), and downregulated the mRNA expression levels of Vim, Acta2, and Smad3 (P < 0.05). Compared with the HSD group, LT treatment also upregulated E-cadherin protein expression while downregulating α-SMA protein expression (P < 0.05), reduced fibrous tissue accumulation in the renal interstitium, and significantly ameliorated renal fibrosis. Conclusion: L-theanine intervention alleviates high-salt diet-induced renal inflammation and delays the progression of renal fibrosis, and its protective mechanism may be associated with the regulation of E-cadherin, Kim-1, vimentin, and α-SMA expression.

Key words: short-term high-salt exposure; renal injury; L-theanine; renal fibrosis

中图分类号:

R151.1

唐芳，唐心月，周林灵，梁婷婷，陈颖，王永怡，宋家乐. L-茶氨酸缓解短期高盐饮食暴露所致C57BL/6J小鼠肾脏纤维化[J]. 食品科学, 2026, 47(19): 1-11 https://doi.org/10.7506/spkx1002-6630-20260511-060

TANG Fang, TANG Xinyue, ZHOU Linling, LIANG Tingting, CHEN Ying, WANG Yongyi, SONG Jiale. L-Theanine Alleviates Renal Fibrosis Induced by Short-Term High-Salt Diet Exposure in C57BL/6J Mice[J]. FOOD SCIENCE, 2026, 47(19): 1-11 https://doi.org/10.7506/spkx1002-6630-20260511-060

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L-茶氨酸缓解短期高盐饮食暴露所致C57BL/6J小鼠肾脏纤维化

L-Theanine Alleviates Renal Fibrosis Induced by Short-Term High-Salt Diet Exposure in C57BL/6J Mice

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