食品科学 ›› 2010, Vol. 31 ›› Issue (5): 121-124.doi: 10.7506/spkx1002-6300-201005028

• 生物工程 • 上一篇    下一篇

富硒双歧杆菌中硒的含量与形态研究

梁 佳1,2,李玉锋1,*,赵金璇1,2,王小燕1,3,李 柏1,胡 巍2,高愈希1,陈春英1,4   

  1. 1.中国科学院高能物理研究所,核分析技术重点实验室,纳米生物效应及安全性重点实验室 2. 山东理工大学生命科学学院 3. 四川师范大学生命科学学院 4. 国家纳米科学中心
  • 收稿日期:2009-06-05 修回日期:2009-11-18 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: 李玉锋 E-mail:liyf@ihep.ac.cn
  • 基金资助:

    国家自然科学基金重大项目(10490180);欧盟第六框架计划项目(FOOD-CT-2006-016253);
    环境化学与生态毒理学国家重点实验室开放课题(KF2008-7)

Selenium Content and Speciation of Selenium-enriched Bifidobacterium

LIANG Jia1,2,LI Yu-feng1,*,ZHAO Jin-xuan1,2,WANG Xiao-yan1,3,LI Bai1,HU Wei2,
GAO Yu-xi1,CHEN Chun-ying1,4   

  1. 1. CAS Key Laboratory of Nuclear Analysis Techniques, CAS Key Laboratory for Biological Effects of Nanomaterials and
    Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;2. School of Life Sciences,
    Shandong University of Technology, Zibo 255049, China;3. School of Life Sciences, Sichuan Normal University, Chengdu
    610068, China;4. National Center for Nanoscience and Technology, Beijing 100190, China
  • Received:2009-06-05 Revised:2009-11-18 Online:2010-03-01 Published:2010-12-29
  • Contact: LI Yu-feng1,*, E-mail:liyf@ihep.ac.cn

摘要:

利用电感耦合等离子质谱(ICP-MS)测定富硒双歧杆菌菌粉及其胶囊制品中硒的含量,研究富硒双歧杆菌细胞壁的破壁方法并利用高效液相色谱- 电感耦合等离子质谱(HPLC-ICP-MS)对富硒双歧杆菌菌粉中硒的形态进行研究。结果表明,富硒双歧杆菌菌粉及其胶囊制品中硒的含量分别为(168.11 ± 2.26)μg/g 和(15.68 ± 0.07)μg/g。与市售富硒产品相比较,富硒双歧杆菌胶囊中硒的含量是富硒大米的100 倍,但不及富硒酵母产品中硒含量的1/10。富硒双歧杆菌产品中硒形态非常复杂,且含有许多不明硒化物,但其主要含硒氨基酸为硒代蛋氨酸,占总硒含量的(59.55 ± 0.13)%。

关键词: 富硒双歧杆菌, 形态分析, 高效液相色谱, 电感耦合等离子质谱

Abstract:

Selenium contents and speciations in powder and capsules containing selenium-enriched Bifidobacterium subjected to cell wall disruption by combinatorial ultrasonic and enzymatic hydrolysis method were determined by high performance liquid chromatography connected with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Results indicated that the selenium contents in powder and capsules containing selenium-enriched Bifidobacterium were (168.11 ± 2.26) μg/g and (15.68 ± 0.07) μg/g, respectively. The selenium content in capsules containing selenium-enriched Bifidobacterium was nearly 100 times as high as that of commercially available selenium-enriched rice, but was less than one tenth of that of commercially available selenium-enriched yeast. A complicated selenium speciation was found in selenium-enriched Bifidobacterium products, and the major amino acid containing selenium was selenomethionine (SeMet), which was up to 59% of total selenium amount.

Key words: selenium-enriched Bifidobacterium, speciation, high performance liquid chromatography, inductively coupled plasma mass spectrometry

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