食品科学 ›› 2010, Vol. 31 ›› Issue (5): 137-140.doi: 10.7506/spkx1002-6300-201005031

• 生物工程 • 上一篇    下一篇

葡萄柚种子提取物对酿酒酵母抑制机理的研究

张 南1,曹思硕2,黄昆仑1,2,罗云波1,郭 星2,元延芳2,许文涛1 , 2 ,*   

  1. 1.中国农业大学食品科学与营养工程学院 2.农业部转基因生物食用安全监督检验测试中心(北京)
  • 收稿日期:2009-03-17 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: 许文涛 E-mail:xuwentao1111@sina.com;cauxwt@yahoo.cn
  • 基金资助:

    国家“863”计划项目(2006AA10Z440);国家自然科学基金项目(308007708)

Preliminary Study on Mechanism of GSE-induced Apoptosis in Saccharomyces cerevisiae

ZHANG Nan1,CAO Si-shuo2,HUANG Kun-lun1,2,LUO Yun-bo1,GUO Xing2,YUAN Yan-fang2,XU Wen-tao1,2,*   

  1. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2. Supervision,
    Inspection and Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing 100083, China
  • Received:2009-03-17 Online:2010-03-01 Published:2010-12-29
  • Contact: XU Wen-tao1,2,* E-mail:xuwentao1111@sina.com;cauxwt@yahoo.cn

摘要:

通过揭示葡萄柚种子提取物(GSE)诱导酿酒酵母凋亡的现象,探讨其对酵母抑制的机理。结果表明:GSE有效抑制酿酒酵母的最低质量浓度为0.13mg/mL。DAPI 和TUNEL 核酸荧光染色表明GSE 能使酿酒酵母细胞核浓缩并导致核裂及DNA 断链。ROS 检测表明GSE 能引起酿酒酵母细胞内活性氧大幅增加。

关键词: 葡萄柚种子提取物, 酿酒酵母, 抑制机理

Abstract:

In this study, grape seed extract (GSE) was used to induce apoptosis of Saccharomyces cerevisia and explore the mechanism of GSE-induced apoptosis. Results indicated that the minimum inhibition concentration of GSE on the growth of Saccharomyces cerevisiae was 0.13 mg/mL. Meanwhile, GSE resulted in nuclear condensation and DNA strand break of Saccharomyces cerevisiae cells according to TUNEL and DAPI staining. Detection of reactive oxygen species (ROS) also revealed that GSE could lead to a larger increase of ROS in Saccharomyces cerevisiae cells.

Key words: grape seed extract (GSE), Saccharomyces cerevisiae, mechanism of GSE-induced

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