食品科学 ›› 2009, Vol. 30 ›› Issue (17): 181-184.doi: 10.7506/spkx1002-6630-200917042

• 生物工程 • 上一篇    下一篇

大豆7S 球蛋白碱性蛋白酶酶解产物的结构表征

段春红,姚晓琳,朱新荣,孙 婉,潘思轶*   

  1. 华中农业大学食品科学技术学院
  • 收稿日期:2009-05-25 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 潘思轶 E-mail:pansiyi@mail.huau.edu.cn
  • 基金资助:

    国家“863”计划项目(2006AA10Z330);
    湖北省新世纪高层次人才工程入选人员科研择优资助项目(鄂人[2003]31号)

Structural Characterization of Alcalase Hydrolysates of Soybean 7S Globulin

DUAN Chun-hong,YAO Xiao-lin,ZHU Xin-rong,SUN Wan,PAN Si-yi*   

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2009-05-25 Online:2009-09-01 Published:2014-04-14
  • Contact: PAN Si-yi* E-mail:pansiyi@mail.huau.edu.cn

摘要:

7S 和11S 球蛋白为大豆蛋白主要组分,对大豆蛋白的物化特性有重要的作用。本研究利用SDS- 聚丙烯酰胺凝胶电泳(SDS-PAGE)、扫描电镜(SEM)、傅立叶红外光谱(FTIR),分析大豆7S 球蛋白及其不同水解度的碱性蛋白酶酶解产物的结构特征;通过疏水性和巯基含量的测定,分析水解度对7S 球蛋白的疏水性和巯基含量的影响。实验结果表明:碱性蛋白酶酶解导致7S 球蛋白的构象发生很大变化,而构象的变化取决于水解的程度。

关键词: 7S球蛋白, 碱性蛋白酶, 水解, 结构表征

Abstract:

Soybean 7S and 11S globulins, two major soybean protein components, play an important role in maintaining the phisico-chemical properties of soybean proteins. The structural properties of both soybean 7S globulin and its hydrolysates were investigated by SDS-PAGE, SEM and FTIR infrared spectroscopy, meanwhile their hydrophobicity and sulfhydryl content were measured. That alcalase-catalyzed hydrolysis led to marked conformational changes was observed in soybean 7S globulin and the degree of change was dependant on the degree of hydrolysis.

Key words: 7S globulin, alcalase, hydrolysis, structural properties

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