食品科学 ›› 2009, Vol. 30 ›› Issue (2 ): 127-129.doi: 10.7506/spkx1002-6630-200902025

• 工艺技术 • 上一篇    下一篇

利用苹果渣发酵生产L-苹果酸工艺研究

张居尚1,杨新文1,雷 萍2 ,* ,孙悦迎2,张文隽2,张 慧2   

  1. 1.陕西省苹果研究发展中心 2.陕西省微生物研究所
  • 收稿日期:2007-12-27 修回日期:2008-09-17 出版日期:2009-01-15 发布日期:2010-12-29
  • 通讯作者: 雷萍 E-mail:wuleiping2529@126.com

Study on L-Malic Acid Production Technology by Submerged Fermentation

ZHANG Ju-shang1,YANG Xin-wen1,LEI Ping2,*,SUN Yue-ying2,ZHANG Wen-jun2,ZHANG Hui2   

  1. (1. Shaanxi Center of Researching and Developing Apple, Xi'an 710016, China;
    2. Shaanxi Institute of Microbiology, Xi'an 710043, China)
  • Received:2007-12-27 Revised:2008-09-17 Online:2009-01-15 Published:2010-12-29
  • Contact: LEI Ping2 E-mail:wuleiping2529@126.com

摘要:

以苹果渣为原料,经过去毒处理,在50L 发酵罐中液体深层发酵生产L- 苹果酸。研究了发酵过程中通气量、温度,以及搅拌转速等因素对苹果渣转化率、L- 苹果酸产率的影响。结果表明,液体深层发酵生产L- 苹果酸的最佳发酵工艺条件为:苹果渣浓度6%,发酵前期通气量(V/V)1:0.15(发酵48h 前)~1:0.4(发酵48h 后),温度30℃(发酵48h 前)~28℃(发酵48h 后),初始pH6.0~6.2,发酵周期108~120h,搅拌转速90r/min,苹果渣的平均转化率66.67%,平均产L- 苹果酸40g/L。

关键词: 苹果渣, 液体深层发酵, 再利用, L-苹果酸

Abstract:

The pomace,which had been detoxified, was used as raw material to produce L-malic acid in 50-L fermentor by submerged fermentation.The fermentation conditions were in vestigated such as the variations of ventilation volume, temperature, pH value and rotation speed, which could affect the conversion rate of pomace and L-malic acid yield. The results showed that the average conversion rate of pomace and the average L-malic acid yield are 66.67% and 40g/L respectively, under the optimal conditions: pomace concentration 6%, ventilation volume (V/V) 1:0.15 (0~48 h) to 1:0.4 (48~120 h), temperature 30 ℃ (0~48 h) to 28 ℃ (48~120 h), initial pH 6.0 to 6.2, fermentation period 108 h to120 h and rotation speed 90 r/min.

Key words: pomace, submerged fermentation, recycle, L-malic acid

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