食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 175-177.doi: 10.7506/spkx1002-6300-200921041

• 生物工程 • 上一篇    下一篇

液体深层发酵条件对银耳菌丝体酸性多糖富集的影响

王瑞雪1,颜 军2,邬晓勇2,何 钢2,张良蕾1,姚尊伟2,苟小军2 ,*   

  1. 1.四川抗菌素工业研究所 2.成都大学中药化学实验室
  • 收稿日期:2009-06-16 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 苟小军 E-mail:jmee@cdu.edu.cn
  • 基金资助:

    四川省中医药管理局科技专项资助项目(2008-01);四川省教育厅科研项目(08ZA173)

Effects of Submerged Fermentation on Acidic Polysaccharides in Tremella fuciformis Mycelia

WANG Rui-xue1,YAN Jun2,WU Xiao-yong2,HE Gang2,ZHANG Liang-lei1,YAO Zun-wei2,,*   

  1. 1. Sichuan Industrial Institute of Antibiotics, Chengdu 610052, China;2. Laboratory for Chemistry of Traditional Chinese
    Medicine, Chengdu University, Chengdu 610106, China
  • Received:2009-06-16 Online:2009-11-01 Published:2010-12-29
  • Contact: GOU Xiao-jun E-mail:jmee@cdu.edu.cn

摘要:

考察液体发酵培养基中不同碳源、碳源用量以及培养基pH 值对银耳菌丝体富集酸性多糖的影响。结果表明:1% 的蔗糖、pH 值为7.0 的培养基有利于银耳菌丝体酸性多糖的富集。并对所得银耳多糖进行DEAE-sepherosefast flow 柱层析,发现菌丝体与子实体相比,多糖组成发生显著改变。

关键词: 银耳, 菌丝体, 酸性多糖, 液体深层发酵

Abstract:

This paper mainly deals with effects of different carbon sources, carbon source amount and medium pH on accumulation of acidic polysaccharides in Tremella fuciformis mycelia during submerged fermentation. Along with this, the fractionation of the polysaccharides by DEAE Sepharose Fast Flow chromatography is also discussed in this paper. Results showed that the fermentation medium containing 1% sucrose at pH 7.0 was conducive to the enrichment of acidic polysaccharides. The distribution of polysaccharide in mycelia and fruit body of Tremella fuciformis revealed a significant change.

Key words: Tremella fuciformis, mycelium, acidic polysaccharide, submerged fermentation

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