食品科学 ›› 2009, Vol. 30 ›› Issue (15 ): 184-187.doi: 10.7506/spkx1002-6630-200915042

• 生物工程 • 上一篇    下一篇

动物双歧杆菌01 耐硒性能及富硒条件的研究

靳志强1,张博文2,李平兰2 ,*   

  1. 1. 长治学院生化系 2. 中国农业大学食品科学与营养工程学院
  • 收稿日期:2009-04-25 出版日期:2009-08-01 发布日期:2010-12-29
  • 通讯作者: 李平兰2 ,* E-mail:lipinglan@cau.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD05A03)

Selenium Tolerance and Enrichment in Bifidobacterium animalis 01

JIN Zhi-qiang1,ZHANG Bo-wen2,LI Ping-lan2,*   

  1. 1. Department of Biochemistry, Changzhi College , Changzhi 046011, China;
    2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2009-04-25 Online:2009-08-01 Published:2010-12-29
  • Contact: LI Ping-lan2,* E-mail:lipinglan@cau.edu.cn

摘要:

对动物双歧杆菌01 的耐硒性能和最适富硒条件进行初步研究。结果显示: 当MRS 培养基中硒(Ⅳ)含量为12μg/ml 时, 即为动物双歧杆菌01 的最大耐硒量;动物双歧杆菌01 富硒最适培养条件为:培养基硒(Ⅳ)浓度8μg/ml,接种量5%,培养6h 时加硒,培养时间为48h;在最适培养条件下进行培养后,其菌体硒含量高达(898 ± 74)μg/g,硒转化率为(28.1 ± 2.4)%。

关键词: 双歧杆菌, 耐硒, 富硒

Abstract:

The selenium tolerance of Bifidobacterium animalis 01 isolated from feces of long-life persons living in Bama area of Guangxi in China was investigated and the conditions for selenium enrichment in this strain were optimized by single-factor and orthogonal array methods. The maximum concentration of selenium in the culture medium was 12 μg/ml for Bifidobacterium animalis 01. The optimal conditions for selenium enrichment in Bifidobacterium animalis 01 were determined as follows: Se(Ⅳ) concentration 8 μg/ml, inoculation amount 5%, adding selenium to the medium at the 6th h of culture, and culture time 48 h. Under these conditions, the selenium content in Bifidobacterium animalis 01reached (898 ± 74) μg/g and the conversion rate of selenium was (28.1 ± 2.4)%.

Key words: Bifidobacterium animalis 01, selenium tolerance, selenium enrichment

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