紫外诱变赤灵芝原生质体选育高产有机锗菌株的研究

doi:10.7506/spkx1002-6630-200915043

食品科学 ›› 2009, Vol. 30 ›› Issue (15 ): 188-192.doi: 10.7506/spkx1002-6630-200915043

紫外诱变赤灵芝原生质体选育高产有机锗菌株的研究

董玉玮，苗敬芝，曹泽虹，高明侠，吕兆启*

徐州工程学院食品工程学院

收稿日期:2009-03-16 出版日期:2009-08-01 发布日期:2010-12-29
通讯作者: 吕兆启* E-mail:kyg@xzit.edu.cn
基金资助:
江苏省教育厅课题 (08KJD18002)；徐州工程学院校级科研项目(XKY2008228)

UV Mutagenization of Protoplast for Screening and Breeding of High-yield Organogemanium-producing Strains of Ganoderma lucidum

DONG Yu-wei，MIAO Jing-zhi，CAO Ze-hong，GAO Ming-xia，LU Zhao-qi*

School of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China

Received:2009-03-16 Online:2009-08-01 Published:2010-12-29
Contact: LU Zhao-qi* E-mail:kyg@xzit.edu.cn

摘要/Abstract

摘要：

对赤灵芝原生质体制备、再生及紫外诱变育种、筛选有机锗高产菌株进行研究。结果表明：赤灵芝原生质体制备与再生的最佳条件为菌龄96h，复合酶液浓度为2% 溶壁酶、1% 蜗牛酶和1% 纤维素酶，温度31℃，时间2h，获得赤灵芝原生质体产量为4.33 × 106 个/ml，再生率达0.00275%。原生质体最佳紫外诱变条件为：15W紫外灯，距离30cm，照射30s，致死率为66.7%。最终选育出3 株高产有机锗诱变株，有机锗含量分别比出发菌株提高了113.58%、105.87%、103.86%。

关键词: 紫外诱变, 原生质体, 赤灵芝, 有机锗

Abstract:

Preparation, regeneration, UV mutagenization of Ganoderma lucidum protoplast were studied to obtain high-yield strains producing organogemanium. Under optimized conditions for the preparation and regeneration of Ganoderma lucidum protoplast including cell age 96 h, lywallzyme concentration 2%, glusulase concentration 1%, cellulase concentration 1%, and temperature 31 ℃for an enzymolysis duration of 2 h, the protoplast yield and regeneration rate were found to be 4.33 × 106 protoplasts/ml and 0.00275%, respectively. When Ganoderma lucidum protoplast was irradiated for 30 s with 15 W ultraviolet lamp 30 cm away from it, the lethality was 66.7% and three high-yield strains were obtained after two passages, the organogemanium contents in whose dried mycelia were higher than that of starting strain by 113.58%, 105.87% and 103.86% separately.

Key words: UV mutagenization, protoplast, Ganoderma lucidum, organogemanium

中图分类号:

S567.31

董玉玮，苗敬芝，曹泽虹，高明侠，吕兆启*. 紫外诱变赤灵芝原生质体选育高产有机锗菌株的研究[J]. 食品科学, 2009, 30(15 ): 188-192.

DONG Yu-wei，MIAO Jing-zhi，CAO Ze-hong，GAO Ming-xia，LU Zhao-qi*. UV Mutagenization of Protoplast for Screening and Breeding of High-yield Organogemanium-producing Strains of Ganoderma lucidum[J]. FOOD SCIENCE, 2009, 30(15 ): 188-192.

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紫外诱变赤灵芝原生质体选育高产有机锗菌株的研究

UV Mutagenization of Protoplast for Screening and Breeding of High-yield Organogemanium-producing Strains of Ganoderma lucidum

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