沙棘籽原花色素组成和结构的研究

食品科学 ›› 2008, Vol. 29 ›› Issue (1): 244-249.

沙棘籽原花色素组成和结构的研究

樊金玲, 罗磊, 要萍, 朱文学

河南科技大学食品与生物工程学院；河北北方学院农林科技学院；河南科技大学食品与生物工程学院河南洛阳471003；河南洛阳471003；河北张家口075000；

出版日期:2008-01-15 发布日期:2011-07-28

Study on Composition and Structure of Proanthocyanidins from Sea Buckthorn (Hippophae rhamnoides) Seeds

FAN Jin-Ling, LUO Lei, YAO Ping, ZHU Wen-Xue

1.College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China; 2.College of Agricultrue and Forestry Science Technology, Hebei North University, Zhangjiakou 075000, China

Online:2008-01-15 Published:2011-07-28

摘要/Abstract

摘要： 采用高效液相色谱-电喷雾串联质谱技术分析鉴定沙棘籽原花色素单体和寡聚体组分,采用SephadexLH-20柱色谱按聚合度大小对沙棘籽多聚体组分进行分级,并通过苄硫醇酸降解反应(简称硫解)对各分级组分的组成结构特征进行分析。共检测到4种单体和8种二聚体:4种单体分别为儿茶素、表儿茶素、棓儿茶素和表棓儿茶素;二聚体包括3种原花青素二聚体、1种原飞燕草素二聚体和4种混合二聚体。并根据ESI提供的质谱信息,分析确定了混合二聚体组成单元的连接顺序。棓儿茶素是构成多聚体各分级组分末端单元的主要组成成分,棓儿茶素和表棓儿茶素(合并计算)是构成各分级组分延伸单元的主要组成成分;平均聚合度为9.1、13.2和17.0,原飞燕草素比例为69.2%、84.6%、87.4%的三个分级组分是多聚体的主要成分,占多聚体总量的74.7%。

关键词: 沙棘籽, 原花色素, 高效液相色谱-电喷雾质谱联用

Abstract: The monomeric and oligomeric proanthocyanidin fractions were analyzed by HPLC-ESI-MASS assay. The polymeric proanthocyanidins were fractionated by Sephadex LH-20 column chromatograph and their chemical constitution were also studied by acid-catalysed degradation in the presence of benzyl mercaptan. Four monomers were identified as catechin, epicatechin, gallocatechin and epigallocatechin. Eight dimers including three dimeric procyanidins, one dimeric prodelphinidin and four mixed dimers were detected and their structures were partly elucidated by the detection of the pseudomolecular ions and main ion fragmentations. The results also showed that the polymers are highly heterogenous, with catechin, epicatechin, gallocatechin and epigallocatechin all being constituent components of both the extension as well as the terminal units. The extension units are mainly gallocatechin, whereas terminal units are mainly gallocatechin and epigallocatechin for all fractions. The polymeric fractions with mDP 9.1, 13.2 and 17.0 represent the three major polymeric fractions accountingfor 74.7% of total polymers.

Key words: sea buckthorn (Hippophae rhamnoides) seeds, proanthocyanidins, HPLC-ESI-MASS

樊金玲, 罗磊, 要萍, 朱文学. 沙棘籽原花色素组成和结构的研究[J]. 食品科学, 2008, 29(1): 244-249.

FAN Jin-Ling, LUO Lei, YAO Ping, ZHU Wen-Xue. Study on Composition and Structure of Proanthocyanidins from Sea Buckthorn (Hippophae rhamnoides) Seeds[J]. FOOD SCIENCE, 2008, 29(1): 244-249.

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