苦丁茶冬青叶多糖的分离纯化及其对羟自由基的清除作用

食品科学 ›› 2008, Vol. 29 ›› Issue (6): 37-40.

• 基础研究 • 下一篇

苦丁茶冬青叶多糖的分离纯化及其对羟自由基的清除作用

王新, 何玲玲, 刘彬

辽宁大学药学院；沈阳化工学院应用化学学院；辽宁大学药学院

出版日期:2008-06-15 发布日期:2011-08-26

Study on Extraction,Purification and Scavenging Activity to Hydroxyl Radicals of Polysaccharides from Leaves of Ilex kudincha C. J. Tseng

WANG Xin, HE Ling-Ling, LIU Bin

1. School of Pharmaceutical Sciences, Liaoning University; 2. School of Applying Chemical, Shenyang Institute of Chemical Technology

Online:2008-06-15 Published:2011-08-26

摘要/Abstract

摘要： 苦丁茶冬青叶经乙醇脱脂、水提醇沉和Sevag法除蛋白,得苦丁茶冬青叶粗多糖KPSⅡ。KPSⅡ经DEAE-纤维素阴离子交换层析柱分离纯化,得到两个纯化多糖组分KPSⅢa和KPSⅢb。KPSⅢa和KPSⅢb经过硫酸水解,还原乙酰化,用气相色谱方法测定了其组成及相对含量。KPSⅢa与KPSⅢb均主要由阿拉伯糖、木糖、甘露糖、半乳糖和葡萄糖组成,其摩尔比分别为10.2:1.0:1.2:8.0:5.4和17.7:1.0:1.1:1.8:4.1。以Fenton反应为产生·OH模型,用紫外分光光度法实验了苦丁茶冬青叶多糖(KPSⅡ、KPSⅢa、KPSⅢb)对模型中产生的·OH的清除作用。结果表明,KPSⅡ、KPSⅢa、KPSⅢb对·OH都具有一定的清除作用,且清除率与多糖的浓度存在一定的量效关系。苦丁茶冬青叶粗多糖KPSⅡ对·OH的清除作用均比纯化多糖(KPSⅢa、KPSⅢb)强。

关键词: 苦丁茶冬青叶, 多糖, 分离纯化, 羟自由基

Abstract: The leaves of Ilex kudincha C. J. Tseng were defatted with 95% EtOH,extracted with water and deproteined three times by Sevag method, and from them, crude polysaccharide noted as KPSⅡwas obtained. The crude polysaccharide KPSⅡ was purified by DEAE-cellulose column, and two refined polysaccharides noted as KPSⅢa and KPSⅢb were obtained. KPS Ⅲa and KPSⅢb were hydrolyzed by 2 mol/L H2SO4, reduced with NaBH4, and were then acetylated with Ac2O. The consequent alditol acetate derivatives were analyzed by GC. The results indicated that KPSⅢa and KPSⅢb are both composed of arabinose, xylose, mannose, galactose and glucose with the molar ratio of 10.2 : 1.0 : 1.2 : 8.0 : 5.4 and 17.7 : 1.0 : 1.1 : 1.8 : 4.1, respectively. The scavenging effects of the polysaccharides of Ilex kudincha C. J. Tseng (KPSⅡ, KPSⅢa and KPSⅢb) on hydroxyl radicals were studied by UV-Visible spectroscopy. The hydroxyl radicals were produced by Fenton reaction. The results showed that KPSⅡ, KPSⅢa and KPSⅢb have the scavenging activity to hydroxyl radicals, in a concentration-dependent manner. The scavenging capacity of the crude polysaccharide of Ilex kudincha C. J. Tseng to hydroxyl radicals is higher than that of the refined polysaccharides.

Key words: leaves of Ilex kudincha C. J. Tseng, polysaccharides, extraction and purification, hydroxyl radicals

王新, 何玲玲, 刘彬. 苦丁茶冬青叶多糖的分离纯化及其对羟自由基的清除作用[J]. 食品科学, 2008, 29(6): 37-40.

WANG Xin, HE Ling-Ling, LIU Bin. Study on Extraction,Purification and Scavenging Activity to Hydroxyl Radicals of Polysaccharides from Leaves of Ilex kudincha C. J. Tseng[J]. FOOD SCIENCE, 2008, 29(6): 37-40.

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苦丁茶冬青叶多糖的分离纯化及其对羟自由基的清除作用

Study on Extraction,Purification and Scavenging Activity to Hydroxyl Radicals of Polysaccharides from Leaves of Ilex kudincha C. J. Tseng

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