水提醇沉法从葵花籽中提取绿原酸

食品科学 ›› 2006, Vol. 27 ›› Issue (1): 159-161.

水提醇沉法从葵花籽中提取绿原酸

郑喜群, 蒋继丰, 刘晓兰, 孙秀云

齐齐哈尔大学生命科学与工程学院；中国农业大学食品科学与营养工程学院

出版日期:2006-01-15 发布日期:2011-09-05

Extraction of Chlorogenic Acid from Sunflower Seeds by Water Solution and Alcohol Sedimentation

ZHENG Xi-Qun, JIANG Ji-Feng, LIU Xiao-Lan, SUN Xiu-Yun

1.College of Life Science and Engineering, Qiqihar University; 2.College of Food Science and Nutritional Engineering, China Agricultural University.

Online:2006-01-15 Published:2011-09-05

摘要/Abstract

摘要： 绿原酸具有抗菌、抗病毒、抗氧化等生物活性。葵花籽富含绿原酸。以水酶法提取葵花籽油脂后分离出的水浸提液为原料,采用醇沉淀法去除浸提液中的多糖,抽滤浓缩滤液后得到绿原酸。多次实验结果表明,浸提液浓缩到固形物含量在13%时,加入浸提浓缩液体积1倍量的95%的乙醇沉淀并过滤掉多糖,反复处理两次,滤液再经真空浓缩得到纯度76%左右的绿原酸。

关键词: 葵花籽, 浸提液, 绿原酸, 多糖

Abstract: Chlorogenic acid has biological activity such as anti-microbes, anti-virus , oxidation prevention and so on. Sunflower seeds are full of chlorogenic acid. This experience extracted chlorogenic acid out of waste aqueous solution from water-enzyme method sunflower seeds oil extraction. The experiments results showed that when concentration of solid substance of the waste aqueous solution was condensated to 13% (W/V)on vacuum, 95% alcohol(1:1,V/V) was added to the condensated solution to sedimentate polysaccharose, sedimentation was centrifugalized, repeated above treatment, and filtrate was collected and condensated on vacuum, then chlorogenic acid crystal of 76% purity was obtained.

Key words: sunflower seeds, aqueous solution, chlorogenic acid, polysaccharose

郑喜群, 蒋继丰, 刘晓兰, 孙秀云. 水提醇沉法从葵花籽中提取绿原酸[J]. 食品科学, 2006, 27(1): 159-161.

ZHENG Xi-Qun, JIANG Ji-Feng, LIU Xiao-Lan, SUN Xiu-Yun. Extraction of Chlorogenic Acid from Sunflower Seeds by Water Solution and Alcohol Sedimentation[J]. FOOD SCIENCE, 2006, 27(1): 159-161.

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