食品科学 ›› 2007, Vol. 28 ›› Issue (11): 271-275.

• 工艺技术 • 上一篇    下一篇

栉孔扇贝营养物的提取与分析

 王晓洁, 刘新生, 孙科深, 杨立红, 姚占娟, 刘明月, 姜芳芳   

  1. 鲁东大学生命科学学院; 鲁东大学校医院; 烟台市质量技术监督局稽查局; 鲁东大学生命科学学院 山东烟台264025; 山东烟台264025; 山东烟台264000;
  • 出版日期:2007-11-15 发布日期:2011-11-22

Extraction and Analysis of Nutritional Components of Chlamys farreri

WANG  Xiao-Jie, LIU  Xin-Sheng, SUN  Ke-Shen, YANG  Li-Hong, YAO  Zhan-Juan, LIU  Ming-Yue, JIANG  Fang-Fang   

  1. 1.College of Life Science, Ludong University, Yantai 264025, China;2.Intramural Hospital, Ludong University, Yantai 264025, China;3.Yantai Bureau of Quality and Technology Supervision, Yantai 264000, China
  • Online:2007-11-15 Published:2011-11-22

摘要: 目的:水提法提取栉孔扇贝中营养物质并测定其多糖、牛磺酸和胆碱的含量。分析营养物提取率与牛磺酸、胆碱、多糖含量的关系。方法:超声波破碎组织法;水提法提取栉孔扇贝中营养物质;苯酚-硫酸显色法测定多糖;乙酰丙酮-甲醛显色法测定牛磺酸;雷氏盐沉淀比色法测定栉孔扇贝中胆碱的含量。结果:当1g组织加30ml水并用超声破碎时,水提物出率最高达23%;多糖在栉孔扇贝水提物中的含量达3.60%~15.09%;牛磺酸的含量为1.13%~1.25%;胆碱的含量为0.31%~0.38%。结论:栉孔扇贝中营养物的提取率受各种提取条件影响较大;营养物中的牛磺酸、胆碱含量受各种提取条件影响不大,而多糖的含量则较大。并且,牛磺酸、胆碱、多糖的含量与营养物提取率不呈正相关。

关键词: 栉孔扇贝, 水提法, 牛磺酸, 胆碱, 多糖

Abstract: Objective: Extracting nutritional material of Chlamys farreri with water-extraction method, and determining the content of its polysaccharide, taurine and choline. Analysing the relationship between the extraction rate of nutritional material and the content of polysaccharide, choline and taurine. Methods: Ultrasonic wave to break tissue, water extraction to get Chlamys farreri nutritional material; phenol-sulfuric acid colorimetry to analyse polysaccharide; formaldehyde - acetylacetone colorimetry to analyse taurine; reinecke salt precipitation colorimetry to analyse choline. Results: The highest rate of water- extraction material is 23% (broken by ultrasonic wave;1g tissue:30 ml water).The polycsaccharide content of nutritional material by water extraction is 3.60%~15.09%, the taurine content is 1.130%~1.252% and the choline content is 0.31%~0.38%. Conclusions: The extraction rate of nutritional material has relation with the different kinds of extraction conditions, but no relation with contents of polysaccharides, cholinergic and taurine.

Key words: Chlamys farreri, water extraction, taurine, choline, polycsaccharide