食品科学 ›› 2004, Vol. 25 ›› Issue (6): 143-146.

• 工艺技术 • 上一篇    下一篇

木耳多糖提取工艺研究

 王金凤   

  1. 哈尔滨商业大学食品工程学院
  • 出版日期:2004-06-15 发布日期:2011-10-24

Studies on the Extracting Technology of Polysaccharide from Auricularia

 WANG  Jin-Feng   

  1. College of Food Technology,Harbin University of Commerce
  • Online:2004-06-15 Published:2011-10-24

摘要: 本文讨论了黑木耳多糖的提取方法,实验结果表明,采用酶法可显著提高多糖提取率,其最佳提取条件为:料水比1:60,浸提温度80℃,浸提时间2.5h,中性蛋白酶用量650IU/g,作用温度43℃,作用时间1.5h,最适pH值7.4;纤维素酶用量375IU/g,作用温度43℃,作用时间1.5h,最适pH值5.3。木耳多糖提取率为4.38%。

关键词: 木耳, 多糖, 蛋白酶, 纤维素酶, 提取

Abstract: The research on the extracting technology of Auricularia polysaccharide was studied in this paper. The results showedthat the extracting rate of polysaccharide was 4.38%.The extracting rate could be increased by enzymatic technology. The besttechnological conditions for the extraction were as follows: the ratio of material and water was 1:60 with a temperature of 80℃and extracting time 2.5h and the amount of Proteinase and cellulase used was 650 IU/g and 375 IU/g respectively with a reactingtime of 1.5h at the temperature of 43℃. The optimum pH of the two respectived enzymes was 7.4 and 5.3.

Key words: Auricularia, polysaccharide, proteinase, cellulase, extraction