菱灵颗粒有效成分的抗肿瘤作用研究

食品科学 ›› 2006, Vol. 27 ›› Issue (9): 253-256.

菱灵颗粒有效成分的抗肿瘤作用研究

牛凤兰, 李晨旭, 王学东, 程舸, 梁睿, 董卿, 王天行

吉林大学公共卫生学院；长春市朝阳区疾病预防控制中心；长春市朝阳区卫生局

出版日期:2006-09-15 发布日期:2011-10-20

Anti-cancer Effects of Ling Ling Pellet

NIU Feng-Lan, Li-Chen-Xu, Wang-Xue-Dong, Cheng-Ge, Liang-Rui, Dong-Qing, Wang-Tian-Xing

1.School of Public Health of Jilin University, Changchun 130021, China; 2.Changchun Center of Disease Prevention and Control, Changchun 130021, China; 3.Bureau of Health of Chaoyang District of Changchun, Changchun 130021, China

Online:2006-09-15 Published:2011-10-20

摘要/Abstract

摘要： 目的:研究菱灵颗粒有效成分—化合物Ⅰ在体外对Hela细胞的作用及其作用机制。方法:采用四甲基偶氮唑盐(MTT)还原法检测化合物Ⅰ对肿瘤生长抑制作用,应用光学显微镜、透射电子显微镜观察细胞形态,流式细胞仪检测细胞周期及细胞凋亡情况。结果:化合物Ⅰ对人宫颈癌细胞生长具有明显的抑制作用及诱导细胞凋亡作用,且这种作用有剂量依赖关系,化合物Ⅰ25、12.5、6.25mg/L剂量组30h抑瘤率为52.04%、34.44%、23.72%,100、50、25、12.5、6.25、3.125mg/L剂量组30h抑瘤率显著正相关,相关系数r=0.9860(p<0.01),IC50值为10.9mg/L。透射电子显微镜对化合物Ⅰ25、12.5、6.25mg/L剂量组受试细胞观察,均出现不同程度细胞凋亡现象,细胞核明显皱缩、染色质趋边凝聚、线粒体空化等特征。流式细胞仪检测发现凋亡峰。结论:化合物Ⅰ对Hela细胞增殖抑制作用明显并且有诱导细胞凋亡作用。

关键词: 菱灵颗粒, Hela细胞, 细胞凋亡

Abstract: Objective : To investigate the effects of Compound I abstracted from ling ling pellet on human Hela cell so as to explore its mechanism. Methods: Growth inhibition was assayed with MTT. The morphological changes of Hela cells induced by compound I were observed with transmission electronic microscope and phase contrast microscope. Cell division cycle distri- bution and apoptosis affected by compound I was determined with flow cytometry. Results: The growth of human Hela cells is significantly inhibited which display strong in a dose and time-dependant manners against and induced apoptosis of Hela cells. Hela cell with estimated IC50 values of 10.9mg/L, concentration of 25, 12.5, 6.25mg/L inhabiting rate is 52.04%, 34.44%, 23.72% after 30h of treatment with compound I. Concentration of 100, 50, 25, 12.5, 6.25, 3.125mg/L inhabiting rate is good correlation (r=0.9860, p<0.01). Expose of Hela cells to 25, 12.5, 6.25mg/L of compound I induced nuclear shrinkage, chromatin conden- sation and margination against nuclear envelope as seen in apoptotic cells. The follow cytometry profile revealed that treatment with different Concentrations compound I inducted apoptosis. Conclusion: Growth of human Hela cells was significantly inhibitory and apoptosis may play an important role in the anti-tumor effect of active compound I.

Key words: ling ling pellet, Hela cell, cell apoptosis

牛凤兰, 李晨旭, 王学东, 程舸, 梁睿, 董卿, 王天行. 菱灵颗粒有效成分的抗肿瘤作用研究[J]. 食品科学, 2006, 27(9): 253-256.

NIU Feng-Lan, Li-Chen-Xu, Wang-Xue-Dong, Cheng-Ge, Liang-Rui, Dong-Qing, Wang-Tian-Xing. Anti-cancer Effects of Ling Ling Pellet[J]. FOOD SCIENCE, 2006, 27(9): 253-256.

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