食品科学 ›› 2008, Vol. 29 ›› Issue (11): 154-156.

• 工艺技术 • 上一篇    下一篇

茶皂素的提取及纯化研究

 李静, 李燕, 党培育   

  1. 上海海洋大学食品学院;
  • 出版日期:2008-11-15 发布日期:2011-12-08

Study on Extraction and Purification of Tea Saponin

 LI  Jing, LI  Yan, DANG  Pei-Yu   

  1. College of Food Science and Technology,Shanghai Ocean University,Shanghai 200090,China
  • Online:2008-11-15 Published:2011-12-08

摘要: 采用超声波法提取茶皂素的工艺,确定最佳的工艺参数为:乙醇浓度80%、料液比1:4、提取温度40℃、超声波功率320W、超声波作用时间60min。最佳工艺条件下的茶皂素粗品得率为18.54%,茶皂素含量60.3%。用硅胶层析法对粗品进行分离纯化。能将茶皂素粗品中的杂质有效地分离,适用于茶皂素的分离纯化。

关键词: 茶皂素, 提取, 超声波, 硅胶柱层析色谱, 纯化

Abstract: In this study,the optimal ultra-sonic extraction conditions of tea saponin(TS) are determined as below:ethanol concentration 80%,ratio of material-liquid 1:4,extraction temperature 40 ℃,ultra-sonic powder 320 W,and extraction time 60 min.Under these conditions,the yield of crude TS is 18.54%,and the content of TS is 60.3%.Then the crude TS was further purificated and separated by silica gel column.The results showed that silica gel chromatograph can efficiently separated TS from impurities,which is suitable to purificate TS.

Key words: tea saponin, extraction, ultra-sonic, silica gel chromatograph, purification