液液微萃取技术在糖与糖醇分析检测中的应用

食品科学 ›› 2008, Vol. 29 ›› Issue (11): 482-486.

液液微萃取技术在糖与糖醇分析检测中的应用

胡磊, 杨冀艳, 盖颖, 王乐, 马莹莹, 任静文, 蒋湘宁

南昌大学中德联合研究院；南昌大学食品科学与技术国家重点实验室；北京林业大学生物科学与技术学院；

出版日期:2008-11-15 发布日期:2011-12-08

Application of Liquid-liquid Microextraction in Sugar and Sugar Alcohol Analysis with GC-MS

HU Lei, YANG Ji-Yan, GAI Ying, WANG Le, MA Ying-Ying, REN Jing-Wen, JIANG Xiang-Ning

1.Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China;2.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;3.College of Life Science and Biotechnology,Beijing Forestry University,Beijing 100083,China

Online:2008-11-15 Published:2011-12-08

摘要/Abstract

摘要： 在糖与糖醇传统前处理方法的基础上,分别研究了糖与糖醇的半微量和微量前处理方法,采用气相色谱质谱联用(GC-MS)技术对衍生化产物进行分析。结果表明,三种前处理方法分析结果相近,但与传统方法相比,后两种方法所用的提取溶剂和衍生化试剂用量大大降低,消耗的时间和人力也大大减少。其中微量前处理方法所用溶剂仅为传统方法的1/200～1/100,处理时间缩短了5倍以上。研究结果表明,毛细管液液微萃取技术(LLME)技术是可行、可靠的,并且具有经济、快速、使用方便等特点。

关键词: 液液微萃取(LLME), 微量衍生化, 糖, 糖醇, 气相色谱质谱联用(GC-MS), 毛细管

Abstract: Based on the traditional pretreatment technique,semi-micro-pretreatment and micro-pretreatment methods of sugar and sugar alcohol were set up respectively.Gas chromatography and mass spectrometry(GC-MS) were used to analyze the derived products.The results showed that the derived sugars and sugar alcohols from the same sample pretreated with the above-mentioned three methods are nearly the same in gradients and contents.However,compared with the above-mentioned traditional method,the amounts of extraction solvent and derivation solvent used in the other two methods greatly decrease and the time and labour consumed by the other two methods also obviously reduce.The amount of solvent consumed by micro-pretreatment technique is 1/200 to 1/100 of that by traditional pretreatment and the time for pretreatment is decreased above 5 times.Compared with the traditional method,liquid-liquid microextraction(LLME) is economical,fast and convenient.

Key words: LLME, micro-derivation, sugar, sugar alcohol, GC-MS, capillary

胡磊, 杨冀艳, 盖颖, 王乐, 马莹莹, 任静文, 蒋湘宁. 液液微萃取技术在糖与糖醇分析检测中的应用[J]. 食品科学, 2008, 29(11): 482-486.

HU Lei, YANG Ji-Yan, GAI Ying, WANG Le, MA Ying-Ying, REN Jing-Wen, JIANG Xiang-Ning. Application of Liquid-liquid Microextraction in Sugar and Sugar Alcohol Analysis with GC-MS[J]. FOOD SCIENCE, 2008, 29(11): 482-486.

[1]	陆敏涛，任廷远，杨建，陆龙发，秦礼康. 花椒精油对链脲佐菌素诱导的糖尿病小鼠糖代谢的影响[J]. 食品科学, 2021, 42(9): 115-122.
[2]	刘慧，吕真真，杨文博，张春岭，刘杰超，焦中高. 不同模拟流通温度对桃果实硬度和糖酸品质的影响[J]. 食品科学, 2021, 42(9): 199-205.
[3]	韩喻，扈莹莹，贺菁蕾，王辉，陈倩，秦立刚. 植物提取物对晚期糖基化终产物抑制机理的研究进展[J]. 食品科学, 2021, 42(9): 233-240.
[4]	牛博，庞广昌，鲁丁强. 花椒麻素的生物功能研究进展[J]. 食品科学, 2021, 42(9): 248-253.
[5]	孙聪，郑明珠，许秀颖，蔡丹，刘美宏，曹勇，刘景圣. 玉木耳多糖对杂粮粥糊化、流变及老化特性的影响[J]. 食品科学, 2021, 42(8): 46-51.
[6]	甄红敏，华晓晗，马俊文，温永平，李延啸，闫巧娟，江正强. 产黄青霉β-甘露聚糖酶的高效表达、性质及应用[J]. 食品科学, 2021, 42(8): 98-105.
[7]	孙金芝，付路静，岳田利，李婧妍，郭春锋. 基于牛乳非蛋白生物标志物定量检测山羊乳中掺加的牛乳[J]. 食品科学, 2021, 42(8): 288-293.
[8]	徐正华，吴家丽，朱克卫，梁玉燊，杨雪娇，赵勇，凌菁，郑思珩，周衡刚，张昆，朱玉珍，曾茂茂. 三文鱼中晚期糖基化终末产物含量测定及产地特征因子分析[J]. 食品科学, 2021, 42(8): 294-298.
[9]	詹慧，宋田源，余刚，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性[J]. 食品科学, 2021, 42(7): 142-148.
[10]	杨忠敏，沈以红，黄先智，王祖文，丁晓雯. 桑叶生物碱对氧化应激小鼠糖脂代谢异常及肝损伤的改善作用[J]. 食品科学, 2021, 42(7): 156-161.
[11]	顾倩，周静，张建梅，尚志，沈婷，杨晓君，胡卫成. 人参皂苷Rh2-壳聚糖纳米粒子制备及对A549细胞的抑制作用[J]. 食品科学, 2021, 42(7): 162-168.
[12]	王丽波，高婧宇，李腾飞，李莲玉，刘博，张洪龑，杨昱，徐雅琴. 硒化蒲公英多糖的制备、结构表征及益生菌促增殖活性[J]. 食品科学, 2021, 42(7): 169-175.
[13]	崔晓谕，郑红英，綦占文，林长青，吴笑天，郭建鹏. 黑蒜对溃疡性结肠炎BALB/c小鼠炎症反应的影响[J]. 食品科学, 2021, 42(7): 185-190.
[14]	袁帅，鲁丁强，庞广昌. ‘印度青’苹果合成与分解代谢通量研究及贮藏温度的优化[J]. 食品科学, 2021, 42(7): 214-219.
[15]	李佳艺，陈赛，刘永乐，俞健，李向红，黄轶群，王发祥. 壳聚糖复合保鲜对草鱼肌肉品质变化的影响[J]. 食品科学, 2021, 42(7): 220-225.