关键词: 副溶血性弧菌, 水产品, 检测

Abstract: In order to improve the detection efficiency of Vibrio parahaemolyticus in aquatic products to meet the need of a system of rapid reaction for food sanitationlas PCR method was established.A primer in connection with t1 gene which is specific for Vibro parahaemolyticus was designed and synthesized,phenol-chloroform method was used to extract DNA,and controls for method specificity were made with Escherichia coli,Salmonella,Shigella,Vibrio alginolyticus.40 aquatic products sampled at random from market were tested by using this method,and the results were evaluated with national standard(GB/T 4789.7 —2003).Results showed that this method is easy for operation,with high level of specificity and the sensitivity of bacterial liquid 103 CFU/ml.The samples with bacteria amount of 1～10 CFU/g can be detected after enrichment of 6 h with a detection cycle of 10 h.Results of this method are more accurate and more reliable compared with national standard.So this method is suitable for rapid detection of Vibrio parahaemolyticus in aquatic products.

Key words: Vibro parahaemolyticus, aquatic products, detection